Duckney Patrick J, Wang Pengwei, Hussey Patrick J
Department of Biosciences, Durham University, Durham, UK.
Health and Life Sciences, Oxford Brookes University, Oxford, UK.
Contact (Thousand Oaks). 2025 May 27;8:25152564251342533. doi: 10.1177/25152564251342533. eCollection 2025 Jan-Dec.
Eukaryotic cells are subdivided into specialised organelle compartments, each with unique physiological environments and functions. Interaction and cross-talk between organelles is inherent to Eukaryotic life, and each organelle is physically interconnected to their surrounding subcellular components including the cytoskeleton and adjacent membrane compartments. In animals and yeast, the mechanisms of organelle interaction have been well characterised and are known to have fundamental importance to life. In contrast, we are only beginning to understand the mechanisms and functions of such interactions in plants. The discovery and ongoing characterisation of the NETWORKED (NET) protein family of plant actin-membrane adaptors has greatly advanced our understanding of the mechanisms of organelle-cytoskeletal interaction. Furthermore, unfolding investigation into the NET proteins has revealed their binding partner, VAMP-ASSOCIATED PROTEIN-27 (VAP27), to be a regulator of organelle tethering and interaction with previously unknown, specialised roles in plants. Research on NET and VAP27 proteins has rapidly increased our knowledge of the mechanisms regulating membrane interaction in plants, their functions in regulating cell structure and organisation, as well as their importance to plant growth, development and stress-response. Here, we discuss the discovery and characterisation of the NET and VAP27 proteins, their regulation of organelle interaction and their functions in plants.
真核细胞被细分为专门的细胞器区室，每个区室都有独特的生理环境和功能。细胞器之间的相互作用和串扰是真核生物生命所固有的，并且每个细胞器都与周围的亚细胞成分（包括细胞骨架和相邻的膜区室）存在物理连接。在动物和酵母中，细胞器相互作用的机制已得到充分表征，并且已知对生命具有至关重要的意义。相比之下，我们才刚刚开始了解植物中这种相互作用的机制和功能。植物肌动蛋白-膜衔接蛋白的网络化（NETWORKED，NET）蛋白家族的发现及持续表征极大地推动了我们对细胞器-细胞骨架相互作用机制的理解。此外，对NET蛋白的深入研究揭示了它们的结合伴侣——VAMP相关蛋白27（VAMP-ASSOCIATED PROTEIN-27，VAP27），它是细胞器拴系和相互作用的调节因子，在植物中具有以前未知的特殊作用。对NET和VAP27蛋白的研究迅速增加了我们对植物中调节膜相互作用机制的了解，它们在调节细胞结构和组织中的功能，以及它们对植物生长、发育和应激反应的重要性。在这里，我们讨论NET和VAP27蛋白的发现与表征、它们对细胞器相互作用的调节以及它们在植物中的功能。
