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敲低MIF-AS1通过调节miR-185-5p/VEGFA轴抑制腰椎间盘突出症的疼痛和炎症。

Knockdown of MIF-AS1 Inhibits Pain and Inflammation in Lumbar Disc Herniation by Modulating the miR-185-5p/VEGFA Axis.

作者信息

Li Guangye, Lin Jili, Zhang Hongtao, Lin Liusu, Wu Mian, Guan Yingjun, Li Junling

机构信息

Department of Orthopedics, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, China.

The Department of Anesthesiology, Zhongshan Torch Development Zone People's Hospital, Zhongshan, China.

出版信息

Global Spine J. 2025 Jun 3:21925682251336711. doi: 10.1177/21925682251336711.

DOI:10.1177/21925682251336711
PMID:40460143
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12133790/
Abstract

Study DesignProspective Study.ObjectiveThe objective of this investigation was to explore the abnormal expression of migration inhibitory factor antisense RNA 1 (MIF-AS1) in lumbar disc herniation (LDH) patients and its relationship to the degree of pain and inflammatory response in LDH, as well as the molecular mechanism of its involvement in LDH.MethodsThis study included 50 patients with LDH. The expression levels of MIF-AS1 were detected by RT-qPCR. The LDH model was constructed in SD rats, and the paw withdrawal mechanical threshold (PWMT) and paw withdrawal thermal latency (PWTL) of LDH rats were detected by behavioral experiments. Enzyme-linked immunosorbent assay (ELISA) was used to detect the concentrations of TNF-α, IL-6, and IL-1β. The targeted regulatory relationships between MIF-AS1 and miR-185-5p, miR-185-5p and VEGFA were verified by a dual-luciferase reporter gene assay.ResultThe expression of MIF-AS1 was up-regulated in LDH patients and correlated with the degree of pain in patients. Low expression of MIF-AS1 reduced the degree of pain and inflammation in LDH rats. In addition, MIF-AS1 may regulate pain and inflammation induced by LDH by modulating the miR-185-5p/VEGFA axis.ConclusionMIF-AS1/miR-185-5p/VEGFA axis may be a therapeutic target for LDH.

摘要

研究设计

前瞻性研究。

目的

本研究旨在探讨腰椎间盘突出症（LDH）患者中迁移抑制因子反义RNA 1（MIF-AS1）的异常表达及其与LDH患者疼痛程度和炎症反应的关系，以及其参与LDH的分子机制。

方法

本研究纳入50例LDH患者。采用RT-qPCR检测MIF-AS1的表达水平。在SD大鼠中构建LDH模型，通过行为学实验检测LDH大鼠的爪部撤离机械阈值（PWMT）和爪部撤离热潜伏期（PWTL）。采用酶联免疫吸附测定（ELISA）检测TNF-α、IL-6和IL-1β的浓度。通过双荧光素酶报告基因测定验证MIF-AS1与miR-185-5p、miR-185-5p与VEGFA之间的靶向调控关系。

结果

MIF-AS1在LDH患者中表达上调，且与患者疼痛程度相关。MIF-AS1低表达减轻了LDH大鼠的疼痛和炎症程度。此外，MIF-AS1可能通过调节miR-185-5p/VEGFA轴来调节LDH诱导的疼痛和炎症。

结论

MIF-AS1/miR-185-5p/VEGFA轴可能是LDH的治疗靶点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df0d/12133790/cbd94244134c/10.1177_21925682251336711-fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df0d/12133790/e1171c0b613a/10.1177_21925682251336711-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df0d/12133790/e0a6f866a34b/10.1177_21925682251336711-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df0d/12133790/e340b92a9e88/10.1177_21925682251336711-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df0d/12133790/c84a8f2b44d0/10.1177_21925682251336711-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df0d/12133790/2d30bb6b700f/10.1177_21925682251336711-fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df0d/12133790/cbd94244134c/10.1177_21925682251336711-fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df0d/12133790/e1171c0b613a/10.1177_21925682251336711-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df0d/12133790/e0a6f866a34b/10.1177_21925682251336711-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df0d/12133790/e340b92a9e88/10.1177_21925682251336711-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df0d/12133790/c84a8f2b44d0/10.1177_21925682251336711-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df0d/12133790/2d30bb6b700f/10.1177_21925682251336711-fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df0d/12133790/cbd94244134c/10.1177_21925682251336711-fig6.jpg

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本文引用的文献

1
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Nat Neurosci. 2024 Oct;27(10):1904-1917. doi: 10.1038/s41593-024-01746-9. Epub 2024 Sep 10.
2
LncRNA MAGI2-AS3 promotes fracture healing through downregulation of miR-223-3p.长链非编码 RNA MAGI2-AS3 通过下调 miR-223-3p 促进骨折愈合。
J Orthop Surg Res. 2024 Jun 22;19(1):370. doi: 10.1186/s13018-024-04850-5.
3
mmu-miR-185 regulates osteoclasts differentiation and migration by targeting Btk.
mmu-miR-185 通过靶向 Btk 调节破骨细胞分化和迁移。
J Gene Med. 2024 May;26(5):e3687. doi: 10.1002/jgm.3687.
4
Identification and validation of ferroptosis-related lncRNA signature in intervertebral disc degeneration.鉴定和验证椎间盘退变中与铁死亡相关的长链非编码 RNA 特征。
Gene. 2024 Jul 1;914:148381. doi: 10.1016/j.gene.2024.148381. Epub 2024 Mar 15.
5
Warm Acupuncture Reduces Pain and Inflammation in Rats with Lumbar Disc Herniation Induced by Autologous Nucleus Pulposus Transplantation via Regulating p38MAPK/NF-κB Pathway.温针灸通过调控 p38MAPK/NF-κB 通路减轻自体髓核移植诱导的腰椎间盘突出症大鼠的疼痛和炎症反应。
J Acupunct Meridian Stud. 2024 Feb 29;17(1):28-37. doi: 10.51507/j.jams.2024.17.1.28.
6
LncRNA SNHG14 activates autophagy via regulating miR-493-5p/Mef2c axis to alleviate osteoporosis progression.长链非编码 RNA SNHG14 通过调控 miR-493-5p/Mef2c 轴激活自噬来减轻骨质疏松症的进展。
Commun Biol. 2023 Nov 4;6(1):1120. doi: 10.1038/s42003-023-05493-8.
7
LncRNA FTX ameliorates neuropathic pain by targeting miR-320a in a rat model of chronic constriction injury.在慢性压迫性损伤大鼠模型中，长链非编码RNA FTX通过靶向miR-320a改善神经性疼痛。
Folia Neuropathol. 2023;61(3):291-300. doi: 10.5114/fn.2023.126846.
8
Immunomorphogenesis in Degenerative Disc Disease: The Role of Proinflammatory Cytokines and Angiogenesis Factors.退行性椎间盘疾病中的免疫形态发生：促炎细胞因子和血管生成因子的作用。
Biomedicines. 2023 Aug 3;11(8):2184. doi: 10.3390/biomedicines11082184.
9
miR-185-5p / ATG101 axis alleviated intestinal barrier damage in intestinal ischemia reperfusion through autophagy.miR-185-5p/ATG101轴通过自噬减轻肠道缺血再灌注中的肠屏障损伤。
Heliyon. 2023 Jul 16;9(7):e18325. doi: 10.1016/j.heliyon.2023.e18325. eCollection 2023 Jul.
10
Neuropilin-1 is essential for vascular endothelial growth factor A-mediated increase of sensory neuron activity and development of pain-like behaviors.神经纤毛蛋白-1 对于血管内皮生长因子 A 介导的感觉神经元活性增加和类痛行为的发展是必需的。
Pain. 2023 Dec 1;164(12):2696-2710. doi: 10.1097/j.pain.0000000000002970. Epub 2023 Jun 27.