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台湾早期成熟罗氏沼虾(淡水长臂大虾)的转录组图谱

Transcriptome profile of early matured Macrobrachium rosenbergii (Giant Freshwater Prawn) from Taiwan.

作者信息

Das Sofia Priyadarsani, Lin Yu-Ru, Wu Yu-Sheng, Liu Chun-Hung, Hu Yeh-Fang, Nan Fan-Hua

机构信息

Department of Aquaculture, National Taiwan Ocean University, Keelung, Taiwan.

Department of Aquaculture, National Pingtung University of Science and Technology, Pingtung, Taiwan.

出版信息

Sci Data. 2025 Jun 3;12(1):932. doi: 10.1038/s41597-025-05206-2.

Abstract

Macrobrachium rosenbergii, the Giant Freshwater Prawn (GFP), is one of the most commonly cultivated prawn species in Asia. The degradation of germplasm and the rise of viral infections like Infectious Precocity Virus (IPV) are two main issues that M. rosenbergii aquaculture has had to deal with recently. Therefore, a transcriptome sequencing of the assembled reads of M. rosenbergii from stage specific IPV infected early matured ovaries has been represented here. Approximately 64.63 million reads from Illumina Novaseq 6000 were generated from three different ovarian stages tissue assembled in Trinity assembler. The M. rosenbergii ovary yielded over 63.21 million clean paired end reads. Assembly generated a total of 108,974 transcripts and 51836 unigenes. Out of the total 51836 unigenes 32.31% (16,753) genes are curated with the help of the NCBI NR database. BUSCO confirmed 97.6% completeness to the high-quality assembly. This present study is an initiative to establish a transcriptome repository for stage specific IPV-infected M. rosenbergii from Taiwan. The information produced will be used as a foundation for transcriptome-based genetic research, which will provide fresh perspectives on the species' precocious puberty and diverse length distribution of this species.

摘要

罗氏沼虾,即淡水大对虾,是亚洲最常养殖的对虾品种之一。种质退化和诸如传染性早熟病毒(IPV)等病毒感染的增加是罗氏沼虾水产养殖最近不得不应对的两个主要问题。因此,本文展示了对来自特定阶段感染IPV的早熟卵巢的罗氏沼虾组装读数进行的转录组测序。在Trinity组装器中组装的三个不同卵巢阶段组织产生了来自Illumina Novaseq 6000的约6463万条读数。罗氏沼虾卵巢产生了超过6321万条干净的双端读数。组装共产生了108974个转录本和51836个单基因。在总共51836个单基因中,32.31%(16753个)基因借助NCBI NR数据库进行了注释。BUSCO确认高质量组装的完整性为97.6%。本研究旨在为来自台湾的特定阶段感染IPV的罗氏沼虾建立一个转录组库。所产生的信息将作为基于转录组的遗传研究的基础,这将为该物种的早熟和不同体长分布提供新的视角。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/13b4/12134350/9248bbd46680/41597_2025_5206_Fig1_HTML.jpg

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