Wu Tingyi, Ji Qianqian, Liao Yanfeng, Zhao Xiaoyu, Hong Ying
Department of Ophthalmology, Peking University Third Hospital, Beijing, China.
Beijing Key Laboratory of Restoration of Damaged Ocular Nerve, Peking University Third Hospital, Beijing, China.
Front Immunol. 2025 May 20;16:1575909. doi: 10.3389/fimmu.2025.1575909. eCollection 2025.
To investigate the influence of aqueous humor cytokines levels on the failure of illuminated microcatheter-assisted circumferential trabeculotomy (MAT) in open-angle glaucoma (OAG) patients.
This was a prospective case series with a follow-up period of 24 months. General information and ocular examinations were recorded. Aqueous humor was collected at the time of surgery. Eight aqueous humor cytokines were analyzed: CCL2, VCAM-1, ICAM-1, IL-6, IL-8, IL-10, CXCL10 and G-CSF. Bioinformatics analysis was used to explore the protein network and the possible pathways related to OAG. Surgical failure was defined as a requirement for glaucoma reoperation or intraocular pressure (IOP) greater than 21mmHg with more than 3 topical antiglaucoma medications at 24-month follow-up.
Sixty-five eyes were enrolled (58 success and 7 failure). The levels of CCL2, ICAM-1, IL-6 and CXCL10 in aqueous humor were significantly higher in the surgical failure group ( = 0.024, 0.002, 0.022 and 0.008, respectively). A higher percentage of secondary glaucoma ( < 0.001), younger age ( = 0.019), worse preoperative BCVA ( = 0.022), higher preoperative IOP ( = 0.022) and more preoperative topical antiglaucoma medications ( = 0.029) were significantly observed in the surgical failure group. Bioinformatics analysis identified 4 hub proteins, including CCL2, CXCL10, IL-6 and CXCR3, and demonstrated the potential role of chemokine signaling pathway in MAT surgical outcome.
Higher concentrations of CCL2, ICAM-1, IL-6 and CXCL10 in the aqueous humor were related to the failure of MAT surgery in OAG patients, and chemokine signaling pathway might be associated with the surgical outcome.
探讨房水细胞因子水平对开角型青光眼（OAG）患者照明微导管辅助环形小梁切开术（MAT）失败的影响。
这是一个随访期为24个月的前瞻性病例系列研究。记录患者的一般信息和眼部检查情况。手术时采集房水。分析8种房水细胞因子：CCL2、血管细胞黏附分子-1（VCAM-1）、细胞间黏附分子-1（ICAM-1）、白细胞介素-6（IL-6）、白细胞介素-8（IL-8）、白细胞介素-10（IL-10）、CXC趋化因子配体10（CXCL10）和粒细胞集落刺激因子（G-CSF）。采用生物信息学分析来探索与OAG相关的蛋白质网络和可能的信号通路。手术失败定义为在24个月随访时需要再次进行青光眼手术，或使用3种以上局部抗青光眼药物后眼压（IOP）仍大于21mmHg。
共纳入65只眼（58例成功，7例失败）。手术失败组房水中CCL2、ICAM-1、IL-6和CXCL10的水平显著更高（分别为P = 0.024、0.002、0.022和0.008）。手术失败组中继发性青光眼的比例更高（P < 0.001）、年龄更小（P = 0.019）、术前最佳矫正视力（BCVA）更差（P = 0.022）、术前眼压更高（P = 0.022）以及术前使用的局部抗青光眼药物更多（P = 0.029），差异均有统计学意义。生物信息学分析确定了4种关键蛋白，包括CCL2、CXCL10、IL-6和CXC趋化因子受体3（CXCR3），并证明趋化因子信号通路在MAT手术结果中具有潜在作用。
房水中较高浓度的CCL2、ICAM-1、IL-6和CXCL10与OAG患者MAT手术失败有关，趋化因子信号通路可能与手术结果相关。
