Kalantari Mahsa, Shahsavani Yasamin, Ezzatabadipour Massood, Shoja Alsadati Hoora, Eslami Maryam, Amanpour Sara
Oral and Dental Diseases Research Center, Department of Oral and Maxillofacial Pathology, Kerman Dental School, Kerman University of Medical Sciences, Kerman, Iran.
Department of Anatomical Sciences, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran.
J Dent Res Dent Clin Dent Prospects. 2025 Mar 31;19(1):33-39. doi: 10.34172/joddd.025.40947. eCollection 2025 Mar.
Extensive research has established the adverse impact of morphine sulfate addiction on the central nervous system. Additionally, studies have shown that the consumption of morphine during pregnancy can impair normal fetal development. This study examined the influence of morphine sulfate on tooth development in rats.
Thirty female Wistar rats were randomly assigned to two groups. The experimental group was given morphine sulfate at a final dose of 0.4 mg/mL, while the control group received only water. Dependence on morphine was verified through the use of naloxone. We examined the effect of morphine sulfate on the development of maxillary first molars in rat embryos on day 19 of gestation, as well as on days 1, 4, 7, and 10 after birth. Two or three rats were separated from each mother and anesthetized using ketamine at 2 mg/kg. Thin sections were prepared from paraffin blocks of the tissue and stained with hematoxylin and eosin. Statistical analyses were conducted with SPSS 20 using ANOVA and post hoc Tukey tests (<0.05).
Research findings indicated that, on the first day after birth, the enamel organ had a significantly greater thickness in the control group (=0.001). Additionally, in the 19-day-old fetus and one-day-old baby groups, the control group had a significantly higher dental papilla thickness than the experimental group (=0.001). Furthermore, the maximum buccolingual width of tooth buds in the control group was significantly greater than that of the experimental group (=0.001). Lastly, the enamel and dentin were significantly thicker in the control group than in the experimental group in the 1-, 4-, 7-, and 10-day-old infant groups (=0.001).
These results suggest that morphine sulfate interfered with the development of the tooth bud and reduced the secretion of enamel and dentin matrix.
广泛的研究已证实硫酸吗啡成瘾对中枢神经系统有不良影响。此外，研究表明孕期使用吗啡会损害胎儿正常发育。本研究考察了硫酸吗啡对大鼠牙齿发育的影响。
将30只雌性Wistar大鼠随机分为两组。实验组给予终浓度为0.4 mg/mL的硫酸吗啡，而对照组仅给予水。通过使用纳洛酮来验证对吗啡的依赖性。我们考察了硫酸吗啡对妊娠第19天大鼠胚胎以及出生后第1、4、7和10天的上颌第一磨牙发育的影响。从每只母鼠中分离出两三只大鼠，用2 mg/kg的氯胺酮麻醉。从组织的石蜡块制备薄片，并用苏木精和伊红染色。使用SPSS 20进行统计分析，采用方差分析和事后Tukey检验（<0.05）。
研究结果表明，出生后第一天，对照组的成釉器厚度显著更大（=0.001）。此外，在19日龄胎儿组和1日龄婴儿组中，对照组的牙乳头厚度显著高于实验组（=0.001）。此外，对照组牙胚的最大颊舌宽度显著大于实验组（=0.001）。最后，在1、4、7和10日龄婴儿组中，对照组的釉质和牙本质明显比实验组厚（=0.001）。
这些结果表明硫酸吗啡干扰了牙胚的发育，并减少了釉质和牙本质基质的分泌。
