Liu Le, Wei Ke, Bao Ke-Xin, Xie Ji-Yu, Wang Xiao-Yi
Key Laboratory of Forest Protection of National Forestry and Grassland Administration, Ecology and Nature Conservation Institute, Chinese Academy of Forestry, Beijing 100091, China.
Key Laboratory of Forest Protection of National Forestry and Grassland Administration, Ecology and Nature Conservation Institute, Chinese Academy of Forestry, Beijing 100091, China.
Ultramicroscopy. 2025 Oct;276:114179. doi: 10.1016/j.ultramic.2025.114179. Epub 2025 May 25.
Anastatus orientalis (Hymenoptera: Eupelmidae) is an egg parasitoid of the internationally quarantined pest Lycorma delicatula, and can be used as a potential biological control agent in practice. Antennae are the important olfactory organs that play a key role in host-parasitoid chemical communication. Therefore, recognition of morphological features is crucial for investigating the olfactory behavior mechanism of parasitic wasps. Here, we observed the ultrastructure of sensilla on the antennae in male and female wasps of A. orientalis using scanning electron microscope. Our results revealed that the antennae of A. orientalis are geniculate, with 13 segments in female wasps and 10 segments in males. The average length of female antennae (1761.17 ± 60.77) μm was shorter than that of males (1883.06 ± 95.68) μm. Ten morphological types of antennal sensilla were found on A. orientalis antennae, including sensilla trichodea (ST), sensilla chaetica (SC), sensilla basiconica (SB), i-type sensilla (IS), corneous sensilla (CS), sensilla campaniformia (Ca), sensilla placodea (Pl), lance sensilla (LS), sensilla coeloconica (Co), and böhm bristles (BBs). Among these, ST had two subtypes (ST I and ST II). SC and Pl were the most abundant sensilla on the antennae of A. orientalis, with wide distribution and large number. Sexual dimorphism was observed in the length, width, abundance, and distribution of sensilla on the antennae. IS were unique to female wasps, suggesting their important role in the recognition and acceptance of host eggs.
东方食卵跳小蜂（膜翅目：旋小蜂科）是国际检疫性害虫斑衣蜡蝉的卵寄生蜂，在实际应用中可作为一种潜在的生物防治剂。触角是重要的嗅觉器官，在寄主与寄生蜂的化学通讯中起关键作用。因此，识别形态特征对于研究寄生蜂的嗅觉行为机制至关重要。在此，我们利用扫描电子显微镜观察了东方食卵跳小蜂雌、雄蜂触角感器的超微结构。我们的结果表明，东方食卵跳小蜂的触角为膝状，雌蜂有13节，雄蜂有10节。雌蜂触角的平均长度（1761.17±60.77）μm短于雄蜂（1883.06±95.68）μm。在东方食卵跳小蜂触角上发现了10种形态类型的触角感器，包括毛形感器（ST）、刺形感器（SC）、锥形感器（SB）、i型感器（IS）、角质感器（CS）、钟形感器（Ca）、板形感器（Pl）、 lance感器（LS）、腔锥感器（Co）和博氏鬃毛（BBs）。其中，ST有两个亚型（ST I和ST II）。SC和Pl是东方食卵跳小蜂触角上数量最多的感器，分布广泛且数量众多。在触角感器的长度、宽度、数量和分布上观察到了性二态性。IS是雌蜂特有的，表明它们在识别和接受寄主卵方面发挥着重要作用。
