Pourrostami Niavol Kasra, Bordoloi Achinta, McKelvey Sean, Suri Rominder P S
Department of Civil and Environmental Engineering, Temple University, Philadelphia, PA, 19122, USA.
Philadelphia Water Department, Planning and Environmental Services, Planning and Research Unit, Philadelphia, PA, 19107, USA.
Environ Sci Pollut Res Int. 2025 May;32(25):15096-15110. doi: 10.1007/s11356-025-36590-2. Epub 2025 Jun 4.
Anaerobic co-digestion of food waste (FW) and sewage sludge (SS) has shown superior performance over anaerobic mono-digestion. However, the fate of antibiotic resistance genes (ARGs) under various co-digestion ratios has been rarely reported to date. Thus, this study investigates the effects of FW:SS ratios on the digester's performance and the fate of ARGs at different FW:SS ratios. The results demonstrated that at a 50:50 FW:SS ratio, 738 mL.g VS of biogas and 393 mL.g VS of methane were produced in the system on day 18. Response surface methodology (RSM) was also used for optimization, showing 42.5% FW is the optimal FW content for maximum biogas and minimum HS production. The distribution of select ARGs (qnrS, tetA, emrB, bla, ampR) was tracked in the liquid and solid fraction of the digestate. Results illustrated a decrease (83-99% reduction) in the overall abundance of the ARGs in the solid fraction after AD. A similar trend was observed for the ARGs in the liquid fractions (65-99% reduction), except for ermB which became 1.74-10.6-fold higher in the final digestate. Also, at 50% FW, the abundance of intl1 increased in the liquid and solid fraction of digestate, indicating increased potential of ARG dissemination via horizontal gene transfer.
食物垃圾（FW）与污水污泥（SS）的厌氧共消化已显示出比厌氧单消化更优越的性能。然而，迄今为止，关于各种共消化比例下抗生素抗性基因（ARG）的归宿鲜有报道。因此，本研究调查了FW:SS比例对消化器性能的影响以及不同FW:SS比例下ARG的归宿。结果表明，在FW:SS比例为50:50时，系统在第18天产生了738 mL.g VS的沼气和393 mL.g VS的甲烷。还采用响应面法（RSM）进行优化，结果表明42.5%的FW是产生最大沼气量和最小HS产量的最佳FW含量。在消化产物的液体和固体部分追踪了选定ARG（qnrS、tetA、emrB、bla、ampR）的分布。结果表明，厌氧消化后固体部分中ARG的总体丰度有所下降（降低了83 - 99%）。液体部分中的ARG也观察到类似趋势（降低了65 - 99%），但ermB除外，其在最终消化产物中升高了1.74 - 10.6倍。此外，在FW含量为50%时，消化产物液体和固体部分中intl1的丰度增加，表明通过水平基因转移传播ARG的潜力增加。
