Prospekta在年龄相关性认知障碍大鼠模型中的促认知功效。
Pro-cognitive efficacy of Prospekta in a rat model of age-associated cognitive impairment.
作者信息
Kardash Elena, Petrova Nataliya, Ganina Ksenia, Tarasov Sergey, Epstein Oleg
机构信息
OOO "NPF "MATERIA MEDICA HOLDING", 47-1 Trifonovskaya str, Moscow, 129272, Russian Federation.
出版信息
BMC Neurosci. 2025 Jun 4;26(1):35. doi: 10.1186/s12868-025-00958-4.
BACKGROUND
Ageing is associated with lower levels of cognitive performance, and novel therapeutics are warranted to correct this defect. Aged rats represent a favourable model of human ageing. The aim of the present study is to examine the effect of a novel neurotropic S100B-targeted drug, Prospekta, on the parameters of delayed alternation in the aged rats compared to responses in the young animals.
METHODS
A spatial working memory task in an operant chamber, where aged and young animals (21 and 2 months old, respectively) needed to alternate between two retractable levers on a trial-by-trial basis to receive a food reward, was tested. We also assessed the Prospekta's effect on expression of some biomarkers of ageing by measuring their levels in the brains of aged rats using Western blot.
RESULTS
Treatment with Prospekta ameliorated cognitive functions, which resulted in a significant reduction of simple and choice reaction times for aged rats. The drug also decreased the number of omission errors at the beginning of the acquisition phase, which indicated the influence of treatment on attention and decision-making process. At the same time, Prospekta did not affect the majority of parameters measured in young animals. The analysis of ageing biomarker expression did not reveal any significant differences between aged rats treated with Prospekta or placebo.
CONCLUSIONS
The findings of this study underscore the potential of Prospekta as a promising pro-cognitive agent for the treatment of age-related cognitive decline. However, further research is imperative to elucidate the precise mechanisms underlying its therapeutic effects and validate its efficacy in vivo.
CLINICAL TRIAL NUMBER
Not applicable.
背景
衰老与认知能力下降有关，因此需要新的治疗方法来纠正这一缺陷。老年大鼠是人类衰老的良好模型。本研究的目的是研究一种新型的靶向神经营养因子S100B的药物Prospekta对老年大鼠延迟交替参数的影响，并与年轻动物的反应进行比较。
方法
在操作性条件反射箱中进行空间工作记忆任务测试，老年和年轻动物（分别为21个月和2个月大）需要在两个可伸缩杠杆之间逐次交替以获得食物奖励。我们还通过蛋白质印迹法测量老年大鼠大脑中某些衰老生物标志物的水平，评估了Prospekta对这些标志物表达的影响。
结果
Prospekta治疗改善了认知功能，老年大鼠的简单反应时间和选择反应时间显著缩短。该药物还减少了习得阶段开始时的遗漏错误数量，这表明治疗对注意力和决策过程有影响。同时，Prospekta对年轻动物测量的大多数参数没有影响。对衰老生物标志物表达的分析未发现用Prospekta或安慰剂治疗的老年大鼠之间有任何显著差异。
结论
本研究结果强调了Prospekta作为一种有前途的促认知药物治疗与年龄相关的认知衰退的潜力。然而，有必要进一步研究以阐明其治疗效果的精确机制并在体内验证其疗效。
临床试验编号
不适用。
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