S-腺苷甲硫氨酸介导的非酶促DNA甲基化导致少量胸腺嘧啶残基的形成以及胞嘧啶转化为5-甲基胞嘧啶。

[Non-enzymatic DNA methylation by S-adenosylmethionine results in the formation of minor thymine residues and 5-methylcytosine from cytosine].

作者信息

Mazin A L, Gimadutdinov O A, Turkin S I, Burtseva N N, Vaniushin B F

出版信息

Mol Biol (Mosk). 1985 Jul-Aug;19(4):903-14.

Abstract

It was found that nonenzymatic DNA methylation proceeds in water solution in the presence of S-adenosylmethionine (AdoMet). The main reaction products are thymine and 5-methylcytosine residues. It was shown that labelled thymine residues are formed also upon DNA incubation in the presence of [methyl-14C]methionine as well as [methyl-14C]cobalamine. Only cytosine reacts with AdoMet resulting in thymine production. AdoMet may be a potential mutagen that induces GC----AT transitions during DNA replication in the cell.

摘要

研究发现，在水相中，非酶促DNA甲基化在S-腺苷甲硫氨酸（AdoMet）存在的情况下进行。主要反应产物是胸腺嘧啶和5-甲基胞嘧啶残基。研究表明，在[甲基-14C]甲硫氨酸以及[甲基-14C]钴胺素存在的情况下，DNA孵育时也会形成标记的胸腺嘧啶残基。只有胞嘧啶与AdoMet反应产生胸腺嘧啶。AdoMet可能是一种潜在的诱变剂，在细胞DNA复制过程中诱导GC向AT的转变。