人类补体成分C3：两种常见基因变体活性C3 S和C3 F的特性

Human complement component C3: characterization of active C3 S and C3 F, the two common genetic variants.

作者信息

Behrendt N

出版信息

Mol Immunol. 1985 Aug;22(8):1005-8. doi: 10.1016/0161-5890(85)90089-6.

Abstract

The two common genetic variants of human C3, C3 S and C3 F, were purified and characterized by SDS-PAGE, agarose gel electrophoresis, isoelectric focusing and amino acid analysis. The difference in electrophoretic mobility between the two variants was conserved after purification, and by isoelectric focusing of the hemolytically active proteins, pI values of 5.86 and 5.81 were determined for C3 S and C3 F, respectively. Any difference in amino acid composition was too small to be detected by amino acid analysis, and the two proteins had the same molecular weight as determined by SDS-PAGE.

摘要

人类补体C3的两种常见基因变体C3 S和C3 F，通过SDS-聚丙烯酰胺凝胶电泳、琼脂糖凝胶电泳、等电聚焦和氨基酸分析进行了纯化和特性鉴定。纯化后，这两种变体在电泳迁移率上的差异得以保留，通过对具有溶血活性的蛋白质进行等电聚焦，分别测定出C3 S和C3 F的等电点值为5.86和5.81。氨基酸组成上的任何差异都太小，无法通过氨基酸分析检测到，并且通过SDS-聚丙烯酰胺凝胶电泳测定，这两种蛋白质具有相同的分子量。