Liu Yunliang, Gao Jing, Huo Yuting, Yang Yang, Yue Daoping, Lin Xianghang
Department of Otolaryngology, Fujian Maternity and Child Health Hospital, College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Medical University, Fuzhou 350001, China.
Department of Otolaryngology, Fujian Children's Hospital, Fuzhou 350001, China.
Anal Methods. 2025 Jun 19;17(24):4961-4969. doi: 10.1039/d5ay00212e.
MiRNA-155 is a reliable biomarker for cancers and allergic rhinitis and also plays a critical role in regulating gene expression and mediating the allergic TH2 process. The accurate determination of miRNA-155 is significant for diagnosing allergic rhinitis, especially for children. Herein, Yb-BiS nanorods were prepared through a hydrothermal method. Gold nanoparticles (AuNPs) were then assembled onto the Yb-BiS nanorods surface to obtain a Yb-BiS/AuNP nanocomposite. Yb-BiS/AuNP nanocomposite was used as a photoactive element for fabricating a near-infrared photoelectrochemical aptasensing platform by assembling an aptamer for the specific recognition of miRNA-155. The variation in photocurrent of the photoelectrochemical aptasensor before and after recognizing miRNA-155 was linearly related to the miRNA-155 concentration in the range of 1.0 fmol L to 1.0 nmol L. The detection limit was calculated to be 0.34 fmol L. The developed photoelectrochemical aptasensor was successfully employed for determining miRNA-155 in serum samples with satisfactory results, indicating that it possessed practical application in the determination of miRNA-155 in real samples.
微小RNA-155是癌症和过敏性鼻炎的可靠生物标志物，在调节基因表达和介导过敏性TH2过程中也起着关键作用。准确测定微小RNA-155对诊断过敏性鼻炎具有重要意义，尤其是对儿童。在此，通过水热法制备了镱铋硫纳米棒。然后将金纳米颗粒（AuNPs）组装到镱铋硫纳米棒表面，得到镱铋硫/金纳米颗粒复合材料。通过组装用于特异性识别微小RNA-155的适配体，将镱铋硫/金纳米颗粒复合材料用作光活性元件，构建近红外光电化学适配体传感平台。识别微小RNA-155前后，光电化学适配体传感器的光电流变化与1.0 fmol/L至1.0 nmol/L范围内的微小RNA-155浓度呈线性关系。计算得出检测限为0.34 fmol/L。所开发的光电化学适配体传感器成功用于测定血清样品中的微小RNA-155，结果令人满意，表明其在实际样品中微小RNA-155的测定中具有实际应用价值。
