hydroalcoholic extract protects against oxidative stress in via DAF-2 and SKN-1.

. () is a South American native species frequently used to treat various symptoms and illnesses, including anemia, fatigue, inflammation, pain, respiratory conditions, kidney and prostate disorders, cancer, diabetes, arthritis, rheumatism, gastric and hepatic dysfunctions, and skin conditions. This study aimed to examine the toxicity attributed to stem bark hydroalcoholic extract and its effects on physiological conditions and stress responses. Different strains of were employed. The LC of extract in N2 wild-type (L4) worms was 15,076.99 ± 1,66 µg/ml. The extract reduced survival to 92.96% at the highest concentration tested (1600 μg/ml; 24 hr), and after 72 hr treatment, concentrations of 800 or 1600 μg/ml decreased survival to 84.7% and 82.8%, respectively. Body development of was also affected by higher concentrations, where only 1600 μg/ml diminished reproduction by 87.88%. Exposure to heat stress reduced survival in a time-dependent manner. Also, protected worms against oxidative damage induced by hydrogen peroxide (HO,) at different concentrations and times and decreased expression of superoxide dismutase-3(SOD-3)(800 μg/ml; 1600 μg/ml), glutathione-S-transferase-4 (GST-4) (800 μg/ml), and catalase (CTL)-1/2/3) (400; 800 μg/ml), suggesting that protection may involve modulation of the decay-accelerating factor-2 (DAF-2) and SKN-1 transcription factor pathways. In conclusion, extract exhibited a low toxicity profile at the concentrations tested and under the conditions used in the study. The extract may serve as a potential antioxidant and stress-protective agent associated with modulation DAF-2 and SKN-1 pathway.