Lobo Larissa Aline Carneiro, Santos Péterson Alves, Lemos Mendes Thais, da Silva Francisco Carlos, Inês Goettert Marcia, Pereira Patrícia
Laboratory of Neuropharmacology and Preclinical Toxicology, Federal University of Rio Grande do Sul, Tuebingen, Germany.
Medicine Department, São Lucas Ji-Paraná University Center (UniSL), Ji-Paraná, Brazil.
J Toxicol Environ Health A. 2025;88(21):873-891. doi: 10.1080/15287394.2025.2514531. Epub 2025 Jun 5.
. () is a South American native species frequently used to treat various symptoms and illnesses, including anemia, fatigue, inflammation, pain, respiratory conditions, kidney and prostate disorders, cancer, diabetes, arthritis, rheumatism, gastric and hepatic dysfunctions, and skin conditions. This study aimed to examine the toxicity attributed to stem bark hydroalcoholic extract and its effects on physiological conditions and stress responses. Different strains of were employed. The LC of extract in N2 wild-type (L4) worms was 15,076.99 ± 1,66 µg/ml. The extract reduced survival to 92.96% at the highest concentration tested (1600 μg/ml; 24 hr), and after 72 hr treatment, concentrations of 800 or 1600 μg/ml decreased survival to 84.7% and 82.8%, respectively. Body development of was also affected by higher concentrations, where only 1600 μg/ml diminished reproduction by 87.88%. Exposure to heat stress reduced survival in a time-dependent manner. Also, protected worms against oxidative damage induced by hydrogen peroxide (HO,) at different concentrations and times and decreased expression of superoxide dismutase-3(SOD-3)(800 μg/ml; 1600 μg/ml), glutathione-S-transferase-4 (GST-4) (800 μg/ml), and catalase (CTL)-1/2/3) (400; 800 μg/ml), suggesting that protection may involve modulation of the decay-accelerating factor-2 (DAF-2) and SKN-1 transcription factor pathways. In conclusion, extract exhibited a low toxicity profile at the concentrations tested and under the conditions used in the study. The extract may serve as a potential antioxidant and stress-protective agent associated with modulation DAF-2 and SKN-1 pathway.
.（）是一种原产于南美洲的物种，常用于治疗各种症状和疾病，包括贫血、疲劳、炎症、疼痛、呼吸系统疾病、肾脏和前列腺疾病、癌症、糖尿病、关节炎、风湿病、胃和肝脏功能障碍以及皮肤疾病。本研究旨在检测 茎皮水醇提取物的毒性及其对生理状况和应激反应的影响。使用了不同品系的 。提取物在 N2 野生型（L4）线虫中的半数致死浓度（LC）为 15,076.99±1,66 μg/ml。在测试的最高浓度（1600 μg/ml；24 小时）下，提取物使存活率降至 92.96%，72 小时处理后，800 或 1600 μg/ml 的浓度分别使存活率降至 84.7%和 82.8%。较高浓度也影响了 的身体发育，其中只有 1600 μg/ml 使繁殖率降低了 87.88%。暴露于热应激以时间依赖性方式降低了 的存活率。此外，在不同浓度和时间下保护线虫免受过氧化氢（HO）诱导的氧化损伤，并降低了超氧化物歧化酶 -3（SOD -3）（800 μg/ml；1600 μg/ml）、谷胱甘肽 -S -转移酶 -4（GST -4）（800 μg/ml）和过氧化氢酶（CTL）-1/2/3）（400；800 μg/ml）的表达，表明这种保护可能涉及对衰变加速因子 -2（DAF -2）和 SKN -1 转录因子途径的调节。总之，在所测试的浓度和本研究使用的条件下， 提取物表现出低毒性特征。该提取物可能作为一种潜在的抗氧化剂和应激保护剂，与调节 DAF -2 和 SKN -1 途径相关。
