Norstrand I F
Neurochem Pathol. 1985 Summer;3(2):73-82. doi: 10.1007/BF02834281.
Because of the importance of adenosine deaminase (ADA) in brain function, a histochemical method for visualizing the enzyme in various areas of the human neuraxis was devised, using an MTT [3-(4,5-dimethyl-thiazolyl-2)-2,5-diphenyltetrazolium bromide] method and glutaraldehyde fixation. Controls consisted of preincubation without the substrate, incubation with omission successively of the substrate, MTT tetrazolium, purine nucleoside phosphorylase (PNP), xanthine oxidase (XO), NaCl, boiling for 20 min prior to fixation and incubation, and of incubation of sections with two powerful inhibitors of the enzyme, i.e., 2'-deoxycoformycin and EHNA [erythro-9-(2-hydroxy-3-nonyl)adenine.HCl]. The positive reaction consisted of the deposition of brownish-purple granules, as well as a diffuse nongranular reaction in the cytoplasm of neurons and glial cells, and in the interstitial spaces. Sections from 15 different areas in four brains were examined by this method. This is the first time that adenosine deaminase has been demonstrated histochemically in the nervous system of humans or of any other species.
由于腺苷脱氨酶（ADA）在脑功能中的重要性，利用MTT [3-(4,5-二甲基-噻唑基-2)-2,5-二苯基溴化四氮唑] 法和戊二醛固定法，设计了一种在人类神经轴不同区域可视化该酶的组织化学方法。对照包括不添加底物的预孵育、依次省略底物、MTT四氮唑、嘌呤核苷磷酸化酶（PNP）、黄嘌呤氧化酶（XO）、NaCl的孵育、固定和孵育前煮沸20分钟，以及用该酶的两种强效抑制剂即2'-脱氧助间型霉素和EHNA [赤型-9-(2-羟基-3-壬基)腺嘌呤·HCl] 孵育切片。阳性反应包括棕紫色颗粒的沉积，以及神经元、胶质细胞的细胞质和间隙中的弥漫性无颗粒反应。用这种方法检查了来自四个大脑15个不同区域的切片。这是首次在人类或任何其他物种的神经系统中通过组织化学方法证实腺苷脱氨酶的存在。
