Yadav Shashank Kumar, Saroha Ankit, Singh Devender, Yadav Pragya, Wankhede Dhammaprakash Pandhari, Singh Gyanendra Pratap, Chinnusamy Viswanathan, Kaur Vikender
Indian Council of Agricultural Research-National Bureau of Plant Genetic Resources (ICAR-NBPGR), New Delhi, 110012, India.
Division of Plant Physiology, ICAR-Indian Agricultural Research Institute, New Delhi, 110012, India.
Plant Cell Rep. 2025 Jun 6;44(7):140. doi: 10.1007/s00299-025-03517-7.
The identified PYL/PYR/RCAR receptor gene family differentially modulates stress-responsive and developmental roles in linseed. This gene family is evolutionarily and functionally conserved in agronomically imperative oilseed crop species. Abscisic acid (ABA) functions as a key phytohormone, exerting significant regulation over diverse cellular activities, notably growth and responses to various environmental stressors. The protonated form of ABA (ABAH) is perceived by intracellular Pyrabactin resistance 1-like (PYR/PYL) proteins, constituting an integral component of the signaling cascade. The PYLs represent one of the largest phytohormone receptor families identified. The receptors have been extensively investigated in numerous plant species. However, the PYL family in oilseed crop species has not been systematically identified at the genome level. This study identified 15 members of the PYL gene family in the linseed, exhibiting a non-uniform distribution across the 15 chromosomes. Based on structural and functional similarities, the phylogenetic analysis divided linseed PYLs (LuPYLs) into three subfamilies. Gene and motif structure analysis of LuPYLs revealed that members of each subfamily share similar gene and motif structures. An evolutionary relationship with PYL members in other oilseed crop species has been established, wherein whole-genome duplication appears to be the primary driving force underlying the expansion of the PYLs. Collinearity segregated PYL genes from all selected species into five distinct clusters, each representing a conserved functional module. The regulation of PYL genes in linseed, including transcriptional analysis of CREs in promoter regions, identification of SNPs, and assessment of miRNA-mediated post-transcriptional regulation, was assessed. Tissue-specific qRT-PCR analysis revealed diverse expression patterns of LuPYL genes, highlighting their roles in plant development and abiotic stress responses. These findings provide valuable insights into how PYL genes contribute to abiotic stress tolerance in flax.
已鉴定出的PYL/PYR/RCAR受体基因家族在亚麻籽中对胁迫响应和发育作用具有不同的调节作用。该基因家族在具有重要农艺价值的油料作物物种中在进化和功能上是保守的。脱落酸（ABA）作为一种关键的植物激素，对多种细胞活动，特别是生长和对各种环境胁迫的反应发挥着重要调节作用。ABA的质子化形式（ABAH）被细胞内类吡咯烷酮抗性1（PYR/PYL）蛋白识别，构成信号级联的一个组成部分。PYLs是已鉴定出的最大的植物激素受体家族之一。这些受体已在众多植物物种中得到广泛研究。然而，油料作物物种中的PYL家族尚未在基因组水平上进行系统鉴定。本研究在亚麻籽中鉴定出15个PYL基因家族成员，它们在15条染色体上分布不均。基于结构和功能的相似性，系统发育分析将亚麻籽PYLs（LuPYLs）分为三个亚家族。LuPYLs的基因和基序结构分析表明，每个亚家族的成员具有相似的基因和基序结构。已建立了与其他油料作物物种中PYL成员的进化关系，其中全基因组复制似乎是PYLs扩展的主要驱动力。共线性分析将所有选定物种的PYL基因分为五个不同的簇，每个簇代表一个保守的功能模块。评估了亚麻籽中PYL基因的调控，包括启动子区域CREs的转录分析、SNP的鉴定以及miRNA介导的转录后调控评估。组织特异性qRT-PCR分析揭示了LuPYL基因的多种表达模式，突出了它们在植物发育和非生物胁迫响应中的作用。这些发现为PYL基因如何促进亚麻对非生物胁迫的耐受性提供了有价值的见解。
