Kumar Sunil, Magotra Ankit, Kumar Narender, Bangar Y C
Department of Livestock Farm Complex, LUVAS, Hisar, 125004, Haryana, India; Department of Animal Genetics and Breeding, LUVAS, Hisar, 125004, Haryana, India.
Department of Animal Genetics and Breeding, LUVAS, Hisar, 125004, Haryana, India.
J Therm Biol. 2025 May;130:104151. doi: 10.1016/j.jtherbio.2025.104151. Epub 2025 May 30.
An investigation was carried out to identify the polymorphism of heat shock protein 70 (HSP70) gene and its expression profiling in Munjal sheep. Blood samples from 40 female Munjal sheep were collected for DNA extraction. After standardization of PCR for ovine HSP 70 gene, sequencing was carried out for single nucleotide polymorphism (SNP) profiling. Multiple sequence alignment was performed to screen SNP in the resource population and subsequently, A to G mutation was observed at c.459 position. Two genotypes viz., AG and AA were obtained. HSP70 expression analysis in different seasons was carried out using enzyme-linked immunosorbent assay (ELISA) kits. After blood collection, serum was separated for studying the gene expression and expression analysis of different groups was compared using t-test. Animals were also grouped in to two groups viz., heat stress susceptible (HSS) and heat stress tolerant (HST) based on heat tolerance coefficient (HTC) i.e. HST having HTC<2.40 and HSS with HTC>2.40. Statistical analysis revealed that expression of HSP70 gene varied markedly among the summer and winter season. Furthermore, a significant variation was observed between adults and lambs (P < 0.05) and between HSS and HST sheep (P < 0.05) for HSP70 gene expression. HSP70 expression was higher in adults in comparison to lambs in both summer and winter season. The identification of heat-resistant animal breeds is crucial for enhancing livestock productivity in the face of impending challenging climatic conditions. Heat shock proteins (HSPs) may serve as potential markers for the selection of these resilient animals.
开展了一项调查，以确定芒贾尔绵羊热休克蛋白70（HSP70）基因的多态性及其表达谱。采集了40只雌性芒贾尔绵羊的血样用于DNA提取。在对绵羊HSP 70基因的PCR进行标准化后，进行测序以分析单核苷酸多态性（SNP）。进行多序列比对以筛选资源群体中的SNP，随后在c.459位置观察到A到G的突变。获得了两种基因型，即AG和AA。使用酶联免疫吸附测定（ELISA）试剂盒对不同季节的HSP70表达进行分析。采血后，分离血清以研究基因表达，并使用t检验比较不同组的表达分析。还根据耐热系数（HTC）将动物分为两组，即热应激敏感（HSS）组和热应激耐受（HST）组，即HTC<2.40的为HST组，HTC>2.40的为HSS组。统计分析表明，HSP70基因的表达在夏季和冬季之间有显著差异。此外，在成年羊和羔羊之间（P<0.05）以及HSS和HST绵羊之间（P<0.05）观察到HSP70基因表达存在显著差异。在夏季和冬季，成年羊的HSP70表达均高于羔羊。面对即将到来的具有挑战性的气候条件，鉴定耐热动物品种对于提高畜牧生产力至关重要。热休克蛋白（HSPs）可能作为选择这些适应性强的动物的潜在标记。
