Tartakovskiĭ I S, Prozorovskiĭ S V, Ditiatkin S Ia, Glazacheva L E
Zh Mikrobiol Epidemiol Immunobiol. 1977 Feb(2):39-42.
The authors obtained a stable variant of the L-forms of Bacillus subtilis capable of exponential growth of the minimal and synthetic medium. An electron-microscopic study of different stages of the L-form formation was carried out by the method of ultra-thin sections. A possibility was shown of the transfer of the L-form formation sign by the method of transformation. DNA isolation from the L-forms by soft lysis considerably facilitated and simplified the genetic analysis of the L-form formation by the transformation method.
作者获得了枯草芽孢杆菌L型的一个稳定变体，其能够在基本培养基和合成培养基中呈指数生长。通过超薄切片法对L型形成的不同阶段进行了电子显微镜研究。通过转化法显示了L型形成特征转移的可能性。通过温和裂解从L型中分离DNA极大地促进并简化了通过转化法对L型形成的遗传分析。
