清醒小鼠的3D经颅超声定位显微镜检查：方案与开源流程
3D transcranial ultrasound localization microscopy in awake mice: protocol and open-source pipeline.
作者信息
Chabouh Georges, Denis Louise, Abioui-Mourgues Myriam, Deloges Jean-Baptiste, Battaglia Jacques, Chavignon Arthur, Heiles Baptiste, Pradier Basile, Bodard Sylvain, Vivien Denis, Orset Cyrille, Couture Olivier
机构信息
Sorbonne Université, UMR 7371 CNRS, Inserm U1146, Laboratoire d'Imagerie Biomedicale, Paris, France.
UNICAEN, Caen Normandie Université,Inserm UMR-S U1237, Physiopathology and Imaging of Neurological Disorders (PhIND), GIP Cyceron, Institut Blood and Brain @ Caen-Normandie (BB@C), Normandie University, Caen, France.
出版信息
Commun Eng. 2025 Jun 6;4(1):102. doi: 10.1038/s44172-025-00415-4.
We present an open-source code for 3D super-resolution ultrasound imaging. Open-3DULM was applied to transcranial imaging in habituated awake mice. Comparative analysis reveals that isoflurane anesthesia induces significant vasodilation and increased cerebral blood flow (CBF) in veins, while only a subset of arteries exhibits these effects compared to the awake state. This method could serve as a reference for developing new types of 3D vascular quantifications, particularly emphasizing the importance of awake and 3D imaging for accurate cerebral blood flow measurement in neuroscience research.
我们展示了一种用于三维超分辨率超声成像的开源代码。Open-3DULM被应用于习惯化清醒小鼠的经颅成像。对比分析表明，异氟烷麻醉会导致显著的血管舒张并增加静脉中的脑血流量（CBF），而与清醒状态相比，只有一部分动脉表现出这些效应。该方法可为开发新型三维血管定量分析提供参考，尤其强调了清醒和三维成像在神经科学研究中准确测量脑血流量的重要性。
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