• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

清醒小鼠的3D经颅超声定位显微镜检查：方案与开源流程

3D transcranial ultrasound localization microscopy in awake mice: protocol and open-source pipeline.

作者信息

Chabouh Georges, Denis Louise, Abioui-Mourgues Myriam, Deloges Jean-Baptiste, Battaglia Jacques, Chavignon Arthur, Heiles Baptiste, Pradier Basile, Bodard Sylvain, Vivien Denis, Orset Cyrille, Couture Olivier

机构信息

Sorbonne Université, UMR 7371 CNRS, Inserm U1146, Laboratoire d'Imagerie Biomedicale, Paris, France.

UNICAEN, Caen Normandie Université,Inserm UMR-S U1237, Physiopathology and Imaging of Neurological Disorders (PhIND), GIP Cyceron, Institut Blood and Brain @ Caen-Normandie (BB@C), Normandie University, Caen, France.

出版信息

Commun Eng. 2025 Jun 6;4(1):102. doi: 10.1038/s44172-025-00415-4.

DOI:10.1038/s44172-025-00415-4
PMID:40481246
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12144258/
Abstract

We present an open-source code for 3D super-resolution ultrasound imaging. Open-3DULM was applied to transcranial imaging in habituated awake mice. Comparative analysis reveals that isoflurane anesthesia induces significant vasodilation and increased cerebral blood flow (CBF) in veins, while only a subset of arteries exhibits these effects compared to the awake state. This method could serve as a reference for developing new types of 3D vascular quantifications, particularly emphasizing the importance of awake and 3D imaging for accurate cerebral blood flow measurement in neuroscience research.

摘要

我们展示了一种用于三维超分辨率超声成像的开源代码。Open-3DULM被应用于习惯化清醒小鼠的经颅成像。对比分析表明，异氟烷麻醉会导致显著的血管舒张并增加静脉中的脑血流量（CBF），而与清醒状态相比，只有一部分动脉表现出这些效应。该方法可为开发新型三维血管定量分析提供参考，尤其强调了清醒和三维成像在神经科学研究中准确测量脑血流量的重要性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/60c0/12144258/ec481158ea68/44172_2025_415_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/60c0/12144258/0bec5938d85e/44172_2025_415_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/60c0/12144258/e2a5d1a90f66/44172_2025_415_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/60c0/12144258/8b5661568c51/44172_2025_415_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/60c0/12144258/164145c24c26/44172_2025_415_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/60c0/12144258/f984ee3e4e31/44172_2025_415_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/60c0/12144258/ec481158ea68/44172_2025_415_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/60c0/12144258/0bec5938d85e/44172_2025_415_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/60c0/12144258/e2a5d1a90f66/44172_2025_415_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/60c0/12144258/8b5661568c51/44172_2025_415_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/60c0/12144258/164145c24c26/44172_2025_415_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/60c0/12144258/f984ee3e4e31/44172_2025_415_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/60c0/12144258/ec481158ea68/44172_2025_415_Fig6_HTML.jpg

相似文献

1
3D transcranial ultrasound localization microscopy in awake mice: protocol and open-source pipeline.清醒小鼠的3D经颅超声定位显微镜检查：方案与开源流程
Commun Eng. 2025 Jun 6;4(1):102. doi: 10.1038/s44172-025-00415-4.
2
Dynamics of isoflurane-induced vasodilation and blood flow of cerebral vasculature revealed by multi-exposure speckle imaging.多曝光散斑成像揭示异氟醚诱导的脑血管扩张和血流动力学。
J Neurosci Methods. 2022 Jan 15;366:109434. doi: 10.1016/j.jneumeth.2021.109434. Epub 2021 Dec 1.
3
Longitudinal Awake Imaging of Mouse Deep Brain Microvasculature with Super-resolution Ultrasound Localization Microscopy.利用超分辨率超声定位显微镜对小鼠深部脑微血管进行纵向清醒成像。
bioRxiv. 2025 Jun 17:2023.09.01.555789. doi: 10.1101/2023.09.01.555789.
4
Dynamic 3D imaging of cerebral blood flow in awake mice using self-supervised-learning-enhanced optical coherence Doppler tomography.使用基于自监督学习增强的光相干多普勒断层成像术对清醒小鼠的脑血流进行动态 3D 成像。
Commun Biol. 2023 Mar 21;6(1):298. doi: 10.1038/s42003-023-04656-x.
5
Evaluation of tumor microvasculature with 3D ultrasound localization microscopy based on 2D matrix array.基于二维矩阵阵列的三维超声定位显微镜对肿瘤微血管的评价。
Eur Radiol. 2024 Aug;34(8):5250-5259. doi: 10.1007/s00330-023-10039-x. Epub 2024 Jan 24.
6
Hemodynamic and neuronal responses to cocaine differ in awake versus anesthetized animals: Optical brain imaging study.清醒和麻醉动物对可卡因的血流动力学和神经元反应不同：光学脑成像研究。
Neuroimage. 2019 Mar;188:188-197. doi: 10.1016/j.neuroimage.2018.11.062. Epub 2018 Dec 1.
7
Cerebral blood flow and BOLD fMRI responses to hypoxia in awake and anesthetized rats.清醒和麻醉大鼠大脑血流及血氧水平依赖性功能磁共振成像对缺氧的反应
Brain Res. 2007 Mar 2;1135(1):186-94. doi: 10.1016/j.brainres.2006.11.097. Epub 2007 Jan 2.
8
Cerebral Microcirculation and Oxygenation Modulation by Transcranial Alternating Current Stimulation in Awake and Anesthetized Mice.清醒和麻醉小鼠经颅交流电刺激对脑微循环和氧合的调节作用
Adv Exp Med Biol. 2023;1438:9-13. doi: 10.1007/978-3-031-42003-0_2.
9
Regional cerebral blood flow and BOLD responses in conscious and anesthetized rats under basal and hypercapnic conditions: implications for functional MRI studies.基础状态和高碳酸血症条件下清醒和麻醉大鼠的局部脑血流及血氧水平依赖性功能磁共振成像反应：对功能磁共振成像研究的启示
J Cereb Blood Flow Metab. 2003 Apr;23(4):472-81. doi: 10.1097/01.WCB.0000054755.93668.20.
10
The effect of continuous positive airway pressure on total cerebral blood flow in healthy awake volunteers.持续气道正压通气对健康清醒志愿者全脑血流量的影响。
Sleep Breath. 2013 Mar;17(1):289-96. doi: 10.1007/s11325-012-0688-0. Epub 2012 Mar 22.

引用本文的文献

1
Progresses and clinical application of super-resolution ultrasound imaging: a narrative review.超分辨率超声成像的进展与临床应用：一篇叙述性综述
Ultrasound J. 2025 Jun 16;17(1):29. doi: 10.1186/s13089-025-00432-6.

本文引用的文献

1
Volumetric Ultrasound Localization Microscopy.容积超声定位显微镜检查法
IEEE Trans Ultrason Ferroelectr Freq Control. 2024 Dec;71(12: Breaking the Resolution Barrier in Ultrasound):1643-1656. doi: 10.1109/TUFFC.2024.3485556. Epub 2025 Jan 8.
2
Underestimation of Flow Velocity in 2-D Super-Resolution Ultrasound Imaging.二维超分辨率超声成像中血流速度的低估
IEEE Trans Ultrason Ferroelectr Freq Control. 2024 Dec;71(12: Breaking the Resolution Barrier in Ultrasound):1844-1854. doi: 10.1109/TUFFC.2024.3416512. Epub 2025 Jan 8.
3
Whole Organ Volumetric Sensing Ultrasound Localization Microscopy for Characterization of Kidney Structure.
全器官容积感应超声定位显微镜用于肾脏结构特征分析。
IEEE Trans Med Imaging. 2024 Nov;43(11):4055-4063. doi: 10.1109/TMI.2024.3411669. Epub 2024 Nov 4.
4
Transthoracic ultrasound localization microscopy of myocardial vasculature in patients.经胸超声定位显微镜检查患者心肌血管。
Nat Biomed Eng. 2024 Jun;8(6):689-700. doi: 10.1038/s41551-024-01206-6. Epub 2024 May 6.
5
Visualization of Renal Glomeruli in Human Native Kidneys With Sensing Ultrasound Localization Microscopy.利用感应超声定位显微镜观察人原代肾脏中的肾小球。
Invest Radiol. 2024 Aug 1;59(8):561-568. doi: 10.1097/RLI.0000000000001061. Epub 2024 Jan 12.
6
Ultrasound localization microscopy.超声定位显微镜。
Z Med Phys. 2023 Aug;33(3):292-308. doi: 10.1016/j.zemedi.2023.02.004. Epub 2023 Jun 15.
7
Super-resolution ultrasound microvascular imaging: Is it ready for clinical use?超分辨率超声微血管成像：它是否已准备好用于临床？
Z Med Phys. 2023 Aug;33(3):309-323. doi: 10.1016/j.zemedi.2023.04.001. Epub 2023 May 20.
8
Sensing ultrasound localization microscopy for the visualization of glomeruli in living rats and humans.超声定位显微镜用于活体大鼠和人类肾小球的可视化。
EBioMedicine. 2023 May;91:104578. doi: 10.1016/j.ebiom.2023.104578. Epub 2023 Apr 20.
9
Ultrasound localization microscopy of the human kidney allograft on a clinical ultrasound scanner.临床超声扫描仪对人肾移植的超声定位显微镜检查。
Kidney Int. 2023 May;103(5):930-935. doi: 10.1016/j.kint.2023.01.027. Epub 2023 Feb 24.
10
Coronary Flow Assessment Using 3-Dimensional Ultrafast Ultrasound Localization Microscopy.使用三维超快速超声定位显微镜评估冠状动脉血流。
JACC Cardiovasc Imaging. 2022 Jul;15(7):1193-1208. doi: 10.1016/j.jcmg.2022.02.008. Epub 2022 Apr 13.