Bakshi Asif, Ben El Kadhi Khaled, Desplan Claude
Center for Genomics and Systems Biology (CGSB), New York University Abu Dhabi, PO Box 129188, Abu Dhabi, United Arab Emirates.
Center for Genomics and Systems Biology (CGSB), New York University Abu Dhabi, PO Box 129188, Abu Dhabi, United Arab Emirates; Department of Biology, New York University, 100 Washington Place, New York, NY 10003, USA.
Curr Opin Neurobiol. 2025 Aug;93:103061. doi: 10.1016/j.conb.2025.103061. Epub 2025 Jun 6.
Generating neuronal diversity from a limited number of neural stem cells is fundamental for the proper functioning of the brain. However, the mechanisms that govern neural fate determination have long been elusive due to the intricate interplay of multiple independent factors that influence a cell's commitment to specific fates. While classical genetics and labeling tools have laid the groundwork for identifying cell types and understanding neural complexity, recent breakthroughs in single-cell transcriptomics and whole-brain connectomics represent a significant advancement in enabling a comprehensive characterization of brain cell types and the underlying mechanisms that encode these neuronal identities. This review focuses on recent developments in our understanding of neural cell fate determination in Drosophila, emphasizing three key mechanisms: spatial patterning, temporal patterning, and neuron-type specific terminal selector transcription factors.
从有限数量的神经干细胞产生神经元多样性是大脑正常运作的基础。然而，由于影响细胞向特定命运分化的多个独立因素之间复杂的相互作用，长期以来，控制神经命运决定的机制一直难以捉摸。虽然经典遗传学和标记工具为识别细胞类型和理解神经复杂性奠定了基础，但单细胞转录组学和全脑连接组学的最新突破代表了一项重大进展，能够全面表征脑细胞类型以及编码这些神经元身份的潜在机制。本综述重点关注我们对果蝇神经细胞命运决定的最新认识进展，强调三个关键机制：空间模式形成、时间模式形成和神经元类型特异性终端选择转录因子。
