Wender M, Kozik M, Goncerzewicz A
Acta Histochem. 1977;58(1):17-30. doi: 10.1016/S0065-1281(77)80103-7.
A histochemical study was performed on the activity of several phosphatases, esterases and oxidoreductases in the spinal cord of cat in the course of the myelinopathy provoked by the cerebrospinal fluid (CSF) exchange. The following results were obtained: 1. Neuroglial cells of the spinal cord react with a slight, focal increase of TPP-ase activity already during the early stage of myelinopathy evoked by repeated withdrawal and reinjecting of CSF into the Cisterna magna of experimental cats. 2. During the late stage of this experimental disease-- the oligo-and astroglia of the spinal cord dtsplay considerably increased activities of ATP-ase, acP, and of various oxidoreductases. 3. The observed morphological and functional (increased enzymic activity) changes of the oligodendroglia as well as the demonstrable damage of myelin sheath, seem to be the results of vasogenous edema. 4. The results of investigations performed did not yield any arguments, which would speak in favour of the assumption, that the changes of the oligodendroglia cells function as the primary cause in the myelinopathy provoked by the CSF exchange, affecting secondary the myelin sheaths. 5. The decissively increased lysosomal acP activity in oligodendroglial cells of the spinal cord investigated during the early stage of this myelinopathy, accompanied by an evidently damaged Golgi apparatus, may be explained when the Golgi zone is not necessarily the only cellular site where acid phosphatase may be performed.
对脑脊液（CSF）交换诱发的猫脊髓髓鞘病过程中几种磷酸酶、酯酶和氧化还原酶的活性进行了组织化学研究。得到以下结果：1. 在实验猫的枕大池反复抽取和重新注入脑脊液诱发的髓鞘病早期，脊髓神经胶质细胞的TPP酶活性有轻微的局灶性增加。2. 在该实验性疾病的晚期，脊髓的少突胶质细胞和星形胶质细胞的ATP酶、酸性磷酸酶和各种氧化还原酶的活性显著增加。3. 观察到的少突胶质细胞的形态和功能（酶活性增加）变化以及髓鞘的明显损伤，似乎是血管源性水肿的结果。4. 所进行的研究结果没有提供任何证据支持以下假设，即少突胶质细胞的变化是脑脊液交换诱发的髓鞘病的主要原因，继而影响髓鞘。5. 在这种髓鞘病早期研究的脊髓少突胶质细胞中，溶酶体酸性磷酸酶活性显著增加，同时高尔基体明显受损，这可以解释为高尔基体区域不一定是进行酸性磷酸酶的唯一细胞部位。
