Popyrina Tatiana N, Aksenova Nadejda A, Shilkova Anastasia A, Minaeva Ekaterina D, Agapov Ilya G, Pal Kunal, Minaev Nikita V, Timashev Peter S, Akopova Tatiana А, Demina Tatiana S
Enikolopov Institute of Synthetic Polymeric Materials RAS, 70 Profsoyuznaya st., Moscow 117393, Russia; Moscow Aviation Institute (National Research University), 4 Volokolamskoe sh., Moscow 125993, Russia.
Institute for Regenerative Medicine, Sechenov First Moscow State Medical University, 8-2 Trubetskaya st., Moscow 119991, Russia.
Int J Biol Macromol. 2025 Jul;318(Pt 3):145022. doi: 10.1016/j.ijbiomac.2025.145022. Epub 2025 Jun 6.
The emulsifying and stabilizing ability of alkylated chitosan was studied in comparison with initial chitosan during the preparation of simple emulsions and in the course of fabrication of polylactide microparticles through an oil/water emulsion solvent evaporation technique. The effect of chitosan chemical structure and solution characteristics (viscosity, surface tension, and hydrophilic lipophilic balance) on the emulsion properties (mean size and size distribution of the dispersed droplets, stability in time, emulsifying activity index, and emulsification stability index) and the polymer microparticles (total yield, mean size and size distribution, surface/volume morphology, and surface chemical composition) was evaluated. It was found that alkylated chitosan had better emulsifying and stabilizing abilities in comparison with unmodified chitosan at all studied concentrations (0.5-2 wt%) with a tendency to stabilization improvement when the concentration increased. The emulsions stabilized with the 2 wt% alkylated versus unmodified chitosan had a smaller average droplet size (48 ± 17 μm vs. 61 ± 23 μm), and the polymeric solutions at this concentration had a higher dynamic viscosity (53 mPa∙s vs. 43 mPa∙s), a lower HLB (22 ± 0.5 vs. 26 ± 0.5), and a surface tension (68 ± 0.5 mN/m vs. 72 mN/m). Alkylated chitosan has also proved to be a better emulsifier for stabilization of biodegradable microparticles giving a higher microparticle total yield (68.8 wt% vs. 46.6 wt%) of particles with a smaller mean size (291 ± 115 μm vs. 306 ± 164 μm).
在通过油/水乳液溶剂蒸发技术制备简单乳液以及制备聚乳酸微粒的过程中，研究了烷基化壳聚糖与初始壳聚糖相比的乳化和稳定能力。评估了壳聚糖化学结构和溶液特性（粘度、表面张力和亲水亲油平衡）对乳液性能（分散液滴的平均尺寸和尺寸分布、随时间的稳定性、乳化活性指数和乳化稳定性指数）以及聚合物微粒（总产率、平均尺寸和尺寸分布、表面/体积形态以及表面化学成分）的影响。结果发现，在所有研究浓度（0.5 - 2 wt%）下，烷基化壳聚糖与未改性壳聚糖相比具有更好的乳化和稳定能力，并且随着浓度增加有稳定性提高的趋势。用2 wt%烷基化壳聚糖与未改性壳聚糖稳定的乳液平均液滴尺寸更小（48 ± 17 μm对61 ± 23 μm），该浓度下的聚合物溶液具有更高的动态粘度（53 mPa∙s对43 mPa∙s）、更低的HLB（22 ± 0.5对26 ± 0.5）和表面张力（68 ± 0.5 mN/m对72 mN/m）。烷基化壳聚糖也已被证明是一种更好的乳化剂，用于稳定可生物降解微粒，可获得更高的微粒总产率（68.8 wt%对46.6 wt%），且微粒平均尺寸更小（291 ± 115 μm对306 ± 164 μm）。
