Metwally Rasha A, El-Sersy Nermeen A, Sikaily Amany El, Sabry Soraya A, Ghozlan Hanan A
Marine Microbiology Lab, National Institute of Oceanography and Fisheries, NIOF, Alexandria, Egypt.
Marine Pollution Lab, National Institute of Oceanography and Fisheries, NIOF, Alexandria, Egypt.
Microb Cell Fact. 2025 Jun 7;24(1):132. doi: 10.1186/s12934-025-02751-8.
Menaquinone (MK), which is also known as vitamin K2, is a kind of lipoquinone that, unlike humans, is biosynthesized in bacteria through a series of steps as a necessary component of their respiratory chain for electron transport among various components of the bacterial cell membrane. MKs are receiving increasing attention as they play several essential biological roles in humans.
In this study, MK was obtained from Kocuria sp. RAM1, characterized using UV absorbance, and validated using nuclear magnetic resonance spectroscopy (NMR) and liquid chromatography-electrospray ionization-quadrupole time-of-flight mass spectrometry (LC-ESI-QTOF-MS). The chemical characterization revealed a total of six MK analogues that were identified and confirmed as MK-1, MK-3, MK-5 (H2), MK-7 (H6), MK-8 (H2), and MK-9. Subsequent to the execution of a significant optimization model, a total KMs of 394.69 µg/ml was obtained, with the MK-1 analog being the dominant one. The antibacterial, anti-inflammatory, antioxidant, anticancer, antidiabetic, and wound-healing activities of MKs were evaluated in vitro. As a result, we discovered that MKs have promising findings on the tested in vitro activities.
Our study was made to evaluate MKs obtained from the Red Sea Kocuria sp. RAM1 to emphasize their significant role in different biological applications. Therefore, from a therapeutic and medicinal perspective, the extracted MKs are interesting for additional in vivo studies.
甲萘醌（MK），也被称为维生素K2，是一种脂醌。与人类不同，它是细菌通过一系列步骤生物合成的，作为其呼吸链中细菌细胞膜各组分间电子传递的必要成分。由于MK在人类中发挥着多种重要的生物学作用，因此受到越来越多的关注。
在本研究中，从科氏菌属RAM1中获得了MK，通过紫外吸收进行表征，并使用核磁共振光谱（NMR）和液相色谱 - 电喷雾电离 - 四极杆飞行时间质谱（LC - ESI - QTOF - MS）进行验证。化学表征显示共鉴定并确认了六种MK类似物，分别为MK - 1、MK - 3、MK - 5（H2）、MK - 7（H6）、MK - 8（H2）和MK - 9。在执行显著的优化模型后，获得的总MKs为394.69μg/ml，其中MK - 1类似物占主导。对MKs的抗菌、抗炎、抗氧化、抗癌、抗糖尿病和伤口愈合活性进行了体外评估。结果，我们发现MKs在测试的体外活性方面有很有前景的发现。
我们的研究旨在评估从红海科氏菌属RAM1中获得的MKs，以强调它们在不同生物学应用中的重要作用。因此，从治疗和药用角度来看，提取的MKs对于进一步的体内研究很有意义。
