Zhao Yifan, Xie Yunyun, Kong Bo, Cao Dongliang, Du Jiahua, Ma Lexun, Long Meiling, Li Liang, Sang Yaru, Cheng Dawei, Lao Huiqi, Zhao Yaqi, Zhang Huajian, Kang Xiangyang, Zhang Pingdong
State Key Laboratory of Efficient Production of Forest Resource, Beijing Forestry University, Beijing, 100083, China.
National Engineering Research Center of Tree Breeding and Ecological Restoration, Beijing Forestry University, Beijing, 100083, China.
BMC Plant Biol. 2025 Jun 7;25(1):773. doi: 10.1186/s12870-025-06824-z.
The artificial induction of unreduced (2n) gametes, due to first division restitution (FDR) or second division restitution (SDR), is an important method to produce triploids in higher plants. It is crucial to evaluate the genetic composition and heterozygosity transmission of induced 2n gametes with different formation mechanisms.
Here, we produced 110 triploids by pollinating the female inflorescence of Populus alba × P. glandulosa with gibberellin-induced 2n pollen of Populus bolleana. The genetic composition and heterozygosity of the induced 2n pollen were inferred from SSR marker analysis of the triploid offspring, using 27 primer pairs distributed across nine Populus chromosomes. This revealed that, among the 110 hybrid triploids, 70 triploids did originate from FDR-type 2n pollen and the other 40 from SDR-type 2n pollen. The FDR-type and SDR-type 2n pollen transmitted a substantially different level of parental heterozygosity (0.7545 and 0.3167, respectively) in P. bolleana. Additionally, compared with the low coefficient of variation (CV = 12.82%) for heterozygosity transmitted by FDR-type 2n pollen, there was much higher coefficient of variation (CV = 48.82%) for that transmitted by SDR-type 2n pollen.
Our findings provide a valuable reference point for bolstering suitable applications of 2n gametes in future research, and can help foster new strategies of triploid breeding in plants.
Not applicable.
通过第一次分裂 restitution（FDR）或第二次分裂 restitution（SDR）人工诱导未减数（2n）配子是高等植物中产生三倍体的重要方法。评估具有不同形成机制的诱导 2n 配子的遗传组成和杂合性传递至关重要。
在此，我们用小叶杨赤霉素诱导的 2n 花粉给银白杨×小叶杨的雌花序授粉，产生了 110 个三倍体。利用分布在九条杨树染色体上的 27 对引物对三倍体后代进行 SSR 标记分析，推断诱导 2n 花粉的遗传组成和杂合性。结果表明，在 110 个杂交三倍体中，70 个三倍体源自 FDR 型 2n 花粉，另外 40 个源自 SDR 型 2n 花粉。FDR 型和 SDR 型 2n 花粉在小叶杨中传递的亲本杂合性水平差异很大（分别为 0.7545 和 0.3167）。此外，与 FDR 型 2n 花粉传递的杂合性低变异系数（CV = 12.82%）相比，SDR 型 2n 花粉传递的杂合性变异系数要高得多（CV = 48.82%）。
我们的研究结果为在未来研究中加强 2n 配子的合适应用提供了有价值的参考点，并有助于培育植物三倍体育种的新策略。
不适用。
