Bhattacharya Shinjini, Kumar Sachin
Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, 781039, Assam, India.
Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, 781039, Assam, India.
Microb Pathog. 2025 Sep;206:107786. doi: 10.1016/j.micpath.2025.107786. Epub 2025 Jun 6.
Duck virus enteritis (DVE) is an infectious and fatal disease that affects numerous species of waterfowl. Duck viral enteritis is caused by Anatid herpesvirus 1 (AnHV-1), which belongs to the Alphaherpesvirinae subfamily. Despite widespread vaccination and the implementation of sophisticated disease management methods, viral propagation has yet to be controlled or avoided. However, live vaccines are always a challenge due to the latency of herpes viruses. As a result, discovering new antiviral medicines and therapy alternatives for DVE is becoming increasingly important. Piperazine is a malleable, medicinally important scaffold that serves as a critical component in several marketed medications with a variety of pharmacological properties. The piperazine was initially developed as an anti-helminthic drug, but it has been repurposed as an effective antiviral drug for some viral infections. In the present study, we evaluated the ability of piperazine to inhibit AnHV-1 replication in vitro and in embryonated chicken eggs. Our data collectively demonstrated that piperazine treatment modulated AnHV-1 replication in chicken embryonic fibroblast (CEF) cells. Furthermore, differential host gene expression was found in piperazine-treated CEF cells after 48 h of incubation post-virus infection. Several genes, including IL-1β, NLRP3, IL18, TNFα, IFN-α, IFN-β, CH25H, and viperin, showed considerable up-regulation upon piperazine treatment in CEF cells. The data implies piperazine modulates AnHV-1 replication by regulating host innate immunity regulatory genes. The findings of the study could be elaborated upon to establish piperazine as an alternative antiviral agent against AnHV-1.
鸭病毒性肠炎（DVE）是一种感染多种水禽的传染性致命疾病。鸭病毒性肠炎由鸭疱疹病毒1型（AnHV-1）引起，该病毒属于α疱疹病毒亚科。尽管广泛接种疫苗并实施了复杂的疾病管理方法，但病毒传播仍未得到控制或避免。然而，由于疱疹病毒的潜伏性，活疫苗始终是一项挑战。因此，寻找针对DVE的新抗病毒药物和治疗方案变得越来越重要。哌嗪是一种可塑性强、具有重要药用价值的骨架结构，是几种具有多种药理特性的上市药物的关键成分。哌嗪最初被开发为抗蠕虫药物，但已被重新用作治疗某些病毒感染的有效抗病毒药物。在本研究中，我们评估了哌嗪在体外和鸡胚中抑制AnHV-1复制的能力。我们的数据共同表明，哌嗪处理可调节鸡胚成纤维细胞（CEF）中AnHV-1的复制。此外，在病毒感染后孵育48小时的哌嗪处理的CEF细胞中发现了宿主基因表达差异。包括IL-1β、NLRP3、IL18、TNFα、IFN-α、IFN-β、CH25H和viperin在内的几个基因在CEF细胞中经哌嗪处理后显示出显著上调。数据表明哌嗪通过调节宿主先天免疫调节基因来调节AnHV-1的复制。该研究结果可进一步阐述，以确立哌嗪作为抗AnHV-1的替代抗病毒剂。
