Chediak J, Maxey B, Telfer M
Am J Clin Pathol. 1977 May;67(5):462-9. doi: 10.1093/ajcp/67.5.462.
Commercially available antiserum to factor VIII was used in several tests to determine whether it might serve as a reference between research laboratories involved in investigation of the factor VIII complex and whether the antiserum might be useful in the screening of large populations of patients with hereditary disorders of factor VIII. In Ouchterlony plates, the antiserum gave a single line of identity with concentrated factor VIII, cryoprecipitate, and human plasma. The antiserum was capable of inhibiting the ristocetin response of normal platelets. Testing antigenic factor VIII by the Laurell technic with the commercial antiserum on plasmas from normal and stressed normal controls, patients with von Willebrand's disease, patients with hemophilia A, and obligate carriers of hemophilia A gave diagnostic and reproducible results.
使用市售的抗凝血因子VIII抗血清进行了多项测试，以确定其是否可作为参与凝血因子VIII复合物研究的各研究实验室之间的参考标准，以及该抗血清是否有助于筛查大量患有遗传性凝血因子VIII疾病的患者。在欧氏免疫扩散板试验中，该抗血清与浓缩凝血因子VIII、冷沉淀物及人血浆形成一条单一的沉淀线。该抗血清能够抑制正常血小板的瑞斯托霉素反应。运用劳雷尔技术，以该市售抗血清检测正常及应激状态下的正常对照者、血管性血友病患者、甲型血友病患者及甲型血友病基因携带者血浆中的抗原性凝血因子VIII，得出了具有诊断价值且可重复的结果。
