Probiotic potential of bacteria associated with the mangrove epiphytic algae and .

The growth of the global population has driven the development of aquaculture as an alternative means of meeting the increasing demand for food. However, this sector faces challenges from pathogen transmission, which can impact both cultured organisms and consumers. Probiotics offer a promising solution by leveraging the antibacterial activity of certain microorganisms against pathogens. Given the limited research on the probiotic potential of microorganisms associated with marine algae, this study aimed to evaluate this potential of bacteria isolated from and , algae from mangroves on the Pacific coast of Colombia. The antibacterial activity of the isolates was evaluated against six fish and shellfish pathogens, leading to the selection of four strains ( sp. AB08, sp. AB17, sp. AN35, and AR37) as probiotic candidates due to their outstanding inhibition of . None of the selected strains formed biofilms, a favorable result from a pathogenicity perspective, while sp. AB08 and AN35 demonstrated notable susceptibility to all tested antibiotics. Additionally, these two strains exhibited broad tolerance to temperature and pH, maintaining viable counts above 10 CFU/mL, characteristics that position them as promising candidates for use as probiotics. However, further studies are needed to better define their probiotic properties, along with evaluations in aquaculture systems to confirm their efficacy and safety.