Han Haoqi, Liu Kaizhen, Wang Yao, Song Mingming, Lian Hongxia, Gao Tengyun, Zhang Liyang, Fu Tong
Henan International Joint Laboratory of Nutrition Regulation and Ecological Raising of Domestic Animal, College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China.
College of Animal Science and Technology, Ningxia University, Yinchuan 750021, China.
Anim Nutr. 2025 Apr 11;21:234-244. doi: 10.1016/j.aninu.2025.02.005. eCollection 2025 Jun.
The aim of the present study was to investigate whether GG (LGG) could be utilized as an effective strategy to promote growth performance of calves and to further study its impact on decreasing diarrhea. A total of 18 healthy Holstein calves (age 8 ± 3 days, weight 42.48 ± 1.04 kg) were raised in a centralized manner to minimize differences and then divided into two groups: one group received LGG supplementation (1 × 10 CFU per calf per day), while the other served as the control (CON). Each group consisted of 9 replicates, with 1 calf per replicate. The experimental period spanned 6 weeks. The experimental results demonstrated an increase of the average daily growth ( = 0.094) and a highly significant reduction in diarrhea ( = 0.001) for the LGG group compared with the CON group. The supplementation of LGG resulted in an increased abundance of endogenous beneficial bacteria in the gut including ( = 0.012) and ( = 0.041) while reducing levels of pathogenic bacteria in the ( = 0.066). These particular groups could serve as biomarker bacteria for calf diarrhea and gut health. The alteration of metabolite production and metabolic pathways were also closely associated with the variation of bacterial composition in the gut microbiome of calves, of which included enrichment of biosynthesis of unsaturated fatty acids, α-linolenic acid and steroid biosynthesis. Overall, LGG administration had a positive impact on improving the intestinal homeostasis, alleviating diarrhea, and enhancing growth performance by modulating gut bacteria and metabolites in pre-weaning calves. Thus, LGG could be a potential alternative to prophylactic antibiotic treatment that would lower associated costs and provide new insights into preventing and treating calf diarrhea.
本研究的目的是调查鼠李糖乳杆菌（LGG）是否可作为促进犊牛生长性能的有效策略，并进一步研究其对减少腹泻的影响。总共18头健康的荷斯坦犊牛（年龄8±3天，体重42.48±1.04千克）集中饲养以尽量减少差异，然后分为两组：一组接受LGG补充剂（每头犊牛每天1×10⁸CFU），而另一组作为对照（CON）。每组有9个重复，每个重复1头犊牛。实验期为6周。实验结果表明，与CON组相比，LGG组的平均日增重增加（P = 0.094），腹泻率显著降低（P = 0.001）。补充LGG导致肠道内源性有益菌丰度增加，包括双歧杆菌属（P = 0.012）和乳杆菌属（P = 0.041），同时降低结肠中病原菌水平（P = 0.066）。这些特定菌群可作为犊牛腹泻和肠道健康的生物标志物细菌。代谢产物产生和代谢途径的改变也与犊牛肠道微生物群细菌组成的变化密切相关，其中包括不饱和脂肪酸生物合成、α-亚麻酸生物合成和类固醇生物合成的富集。总体而言，施用LGG对改善断奶前犊牛的肠道内环境稳定、减轻腹泻和提高生长性能具有积极影响，方法是调节肠道细菌和代谢产物。因此，LGG可能是预防性抗生素治疗的潜在替代方法，这将降低相关成本，并为预防和治疗犊牛腹泻提供新的见解。
