Yu Jun, Duan Wu, Zhang Jing, Hao Ming, Li Jie, Zhao Ruopu, Wu Weikang, Sua Heng Han, Jun Hieng Kiat, Liu Yilun, Lu Yang, Liu Yang, Liu Sida
Laboratory for Multiscale Mechanics and Medical Science, SV LAB, School of Aerospace, Xi'an Jiaotong University, Xi'an, 710049, China.
Department of Endocrinology, Qilu Hospital of Shandong University, Jinan, 250012, Shandong, China.
Mater Today Bio. 2025 May 10;32:101840. doi: 10.1016/j.mtbio.2025.101840. eCollection 2025 Jun.
In diabetic patients, hyperglycemia-induced elevated reactive oxygen species (ROS) accumulation severely impairs chronic wound healing by causing cellular component oxidation, inducing DNA damage, triggering cell death, exacerbating inflammatory responses, disrupting vascular endothelial function, reducing local blood supply, and inhibiting angiogenesis. This cascade results in a vicious cycle that delays the healing process. In this study, we developed a novel multifunctional composite dressing by depositing a transition-metal catalytic coating onto a superhydrophobic polydimethylsiloxane layer via magnetron sputtering. Two coatings were developed based on vanadium-ruthenium-boron (VRuB) intermetallic and VRu intermetallic compounds, which functioned as intermetallic compounds and exhibited various enzyme-like activities. The VRuB coating exhibited particularly prominent catalase-like activity (maximal reaction velocity (V) of 48.53 × 10 M s; turnover number of 7.66 s). Experimental characterizations and theoretical calculations revealed that B incorporation significantly improved catalytic performance. The artificial enzyme spray-coating process retained superhydrophobicity at the wound-contacting interface while enhancing the ROS-scavenging capabilities. Biological experiments demonstrated that the coating exhibited excellent biocompatibility and effective ROS-scavenging characteristics. These benefits were attributed to its synergistic properties, including its anti-adhesion characteristics, unidirectional drainage, moisturizing effects, and ROS elimination, which collectively promoted wound healing, especially for diabetic wound healing. The material showed promise for other applications requiring localized ROS scavenging while maintaining interfacial biomechanical properties.
在糖尿病患者中，高血糖诱导的活性氧（ROS）积累增加，通过引起细胞成分氧化、诱导DNA损伤、触发细胞死亡、加剧炎症反应、破坏血管内皮功能、减少局部血液供应和抑制血管生成，严重损害慢性伤口愈合。这种级联反应导致恶性循环，延迟愈合过程。在本研究中，我们通过磁控溅射在超疏水聚二甲基硅氧烷层上沉积过渡金属催化涂层，开发了一种新型多功能复合敷料。基于钒-钌-硼（VRuB）金属间化合物和VRu金属间化合物开发了两种涂层，它们作为金属间化合物发挥作用，并表现出各种类酶活性。VRuB涂层表现出特别突出的过氧化氢酶样活性（最大反应速度（V）为48.53×10 M s；周转数为7.66 s）。实验表征和理论计算表明，硼的掺入显著提高了催化性能。人工酶喷涂过程在伤口接触界面保持了超疏水性，同时增强了ROS清除能力。生物学实验表明，该涂层具有优异的生物相容性和有效的ROS清除特性。这些益处归因于其协同特性，包括抗粘附特性、单向引流、保湿效果和ROS消除，这些共同促进了伤口愈合，尤其是糖尿病伤口的愈合。该材料在需要局部清除ROS同时保持界面生物力学性能的其他应用中显示出前景。
