Qu-1：一种可用于转化和再生的双单倍体细胞系，具有参考基因组序列，用于[具体物种]中的遗传和功能研究。（注：原文中“in.”后面似乎缺少具体内容）

Qu-1: a transformation-and regeneration-amenable doubled haploid cell line with a reference genome sequence for genetic and functional studies in .

作者信息

Liu Caixia, Wang Meng, Fan Erqin, Wang Sui, Li Shuang, Fu Pengyue, Liu Yi, Liu Yuhang, Wang Junhui, Sederoff Heike W, You Xiangling, Yang Chuanping, Sederoff Ronald R, Chen Su, Qu Guanzheng

机构信息

State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, China.

State Key Laboratory of Utilization of Woody Oil Resource, Central South University of Forestry and Technology, Changsha 410004, China.

出版信息

For Res (Fayettev). 2025 Apr 29;5:e008. doi: 10.48130/forres-0025-0008. eCollection 2025.

DOI:10.48130/forres-0025-0008

PMID:40487538

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12141832/

Abstract

Plant suspension homogeneous cells are invaluable materials for investigating molecular mechanisms underlying various biological processes. In this study, we established and characterized a doubled haploid cell line from × , designated as Qu-1. This cell line exhibited high viability and dispersibility under suspension culture conditions and retained the ability to regenerate into whole plants. -mer analysis confirmed the homozygous genome of Qu-1, and a chromosome-level genome assembly was subsequently achieved by using PacBio sequencing. Additionally, we established an efficient transient transformation protocol using PEG-mediated protoplasts and a stable -mediated transformation system for Qu-1. To explore the mutagenesis potential of this cell line, we treated the cell line with ethyl methanesulfonate (EMS) and performed genome resequencing to identify mutation sites. Overall, Qu-1 represents the first poplar cell line with a homozygous genome and is as a powerful tool for molecular biology research in woody plants.

摘要

植物悬浮均匀细胞是研究各种生物过程分子机制的宝贵材料。在本研究中，我们从×建立并鉴定了一个双单倍体细胞系，命名为Qu-1。该细胞系在悬浮培养条件下表现出高活力和分散性，并保留了再生为完整植株的能力。-mer分析证实了Qu-1的纯合基因组，随后通过PacBio测序实现了染色体水平的基因组组装。此外，我们为Qu-1建立了一种使用PEG介导原生质体的高效瞬时转化方案和一种稳定的-介导转化系统。为了探索该细胞系的诱变潜力，我们用甲磺酸乙酯（EMS）处理该细胞系并进行基因组重测序以鉴定突变位点。总体而言，Qu-1代表了第一个具有纯合基因组的杨树细胞系，是木本植物分子生物学研究的有力工具。