Kerdput Vichununt, Yodkamol Vararut, Sookprasong Mattika, Wongwadhunyoo Wongsakorn, Khunsri Iyacoob, Masrinoul Promsin, Wisedchanwet Trong, Uthailak Naphatsamon, Limpanont Yanin, Reamtong Onrapak, Adisakwattana Poom, Thawornkuno Charin
Department of Molecular Tropical Medicine and Genetics, Faculty of Tropical Medicine, Mahidol University, Bangkok 10400, Thailand.
The Monitoring and Surveillance Center for Zoonotic Diseases in Wildlife and Exotic Animals, Faculty of Veterinary Science, Mahidol University, Nakhon Pathom 73170, Thailand.
Biotechnol Rep (Amst). 2025 May 8;46:e00897. doi: 10.1016/j.btre.2025.e00897. eCollection 2025 Jun.
This study investigated the potential of using laying hens to produce rabies immunoglobulin (RIG) due to concerns about an African Horse Sickness outbreak in 2020. The hens were immunized with rabies vaccine, resulting in the production of Immunoglobulin Y (IgY) antibodies in their egg yolks. The study found that using polyethylene glycol 6000 (PEG) was the most effective method for extracting anti-rabies IgY from egg yolks in terms of amount (1.87 mg of IgY from one mL of egg yolk), purity (83 %), and cost-effectiveness. Further purification through affinity chromatography increased the purity to 95 %, as confirmed by 1D GeLC-MS/MS, showing a purity of up to 98 %. In addition, IgY extracted using PEG demonstrated the highest neutralizing activity compared to the caprylic acid and ammonium sulfate methods. It was estimated that only 8 eggs are needed to produce a vial of RIG with the same potency as equine RIG (1000 IU/vial).
由于对2020年非洲马瘟疫情的担忧，本研究调查了利用蛋鸡生产狂犬病免疫球蛋白（RIG）的潜力。给这些母鸡接种狂犬病疫苗，使其蛋黄中产生免疫球蛋白Y（IgY）抗体。该研究发现，就产量（每毫升蛋黄可提取1.87毫克IgY）、纯度（83%）和成本效益而言，使用聚乙二醇6000（PEG）是从蛋黄中提取抗狂犬病IgY最有效的方法。通过亲和层析进一步纯化后，经一维凝胶液相色谱-串联质谱（1D GeLC-MS/MS）确认，纯度提高到了95%，最高可达98%。此外，与辛酸法和硫酸铵法相比，用PEG提取的IgY具有最高的中和活性。据估计，只需8枚鸡蛋就能生产出一瓶效力与马源RIG（1000 IU/瓶）相同的RIG。
