锂诱导人粒细胞释放叶酸结合蛋白。

Li+-induced release of the folate binding protein from human granulocytes.

作者信息

Holm J, Hansen S I, Lyngbye J

出版信息

Scand J Haematol. 1985 Jul;35(1):109-14. doi: 10.1111/j.1600-0609.1985.tb00809.x.

DOI:10.1111/j.1600-0609.1985.tb00809.x

Abstract

Release of folate binding protein from human leucocytes suspended in isotonic NaCl was studied in experiments where folate binding activity of the suspending medium was determined by equilibrium dialysis (pH 7.4, 37 degrees C) against 3H-folate. Release from leucocytes containing particularly high concentrations of folate binding protein was significantly increased in the presence of 70 mmol/l Li+. The stimulated release was apparently not an osmotic phenomenon since no effect was observed with isomolar Na+/K+. The released protein resembled the folate binding protein in leucocyte lysate as to characteristics of radioligand binding, ionic properties and molecular size. Hence no transformation of the protein seemed to occur during Li+-induced release.

摘要

在通过平衡透析（pH 7.4，37摄氏度）针对³H-叶酸测定悬浮介质的叶酸结合活性的实验中，研究了从悬浮于等渗氯化钠中的人白细胞中释放叶酸结合蛋白的情况。在70 mmol/l Li⁺存在的情况下，从含有特别高浓度叶酸结合蛋白的白细胞中的释放显著增加。这种刺激释放显然不是渗透现象，因为等摩尔的Na⁺/K⁺未观察到效果。释放的蛋白质在放射性配体结合特性、离子性质和分子大小方面类似于白细胞裂解物中的叶酸结合蛋白。因此，在Li⁺诱导的释放过程中似乎没有发生蛋白质的转化。