羧甲基壳聚糖-十六烷基三甲基溴化铵自组装体的合成、表征及药物递送应用
Synthesis, characterization, and drug delivery application of carboxymethyl chitosan-CTAB self-assembling.
作者信息
Dong Lijun, Hong Ge, Zhao Xin, Jiang Mengmeng, Wei Jianrong, Li Yanyan
机构信息
College of Life Sciences, Hebei University, Baoding, Hebei, 071002, China.
Tianjin Key Laboratory of Biomedical Materials, Institute of Biomedical Engineering, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin, 300192, China.
出版信息
Naunyn Schmiedebergs Arch Pharmacol. 2025 Jun 9. doi: 10.1007/s00210-025-04346-x.
In this study, self-assembly vesicles based on carboxymethyl chitosan (CMCS) were prepared for insulin delivery. The self-assembly behavior of mixed systems consisting of CMCS and the cetyl trimethyl ammonium bromide (CTAB) was investigated. The resulting CMCS/CTAB vesicles were nearly spherical in shape, with the majority ranging around 100 nm in diameter. The toxicity profile of CMCS/CTAB vesicles was evaluated in vitro via methyl thiazolyl tetrazolium (MTT) assay. The results indicated that the stability of CMCS/CTAB vesicles was enhanced by increasing the degree of substitution (DS) of CMCS or by reducing the molecular weight (MW) of CMCS. Additionally, we observed that vesicle stability improved with decreased temperature. The insulin loading capacity and encapsulation efficiency decreased as the MW increased. Moreover, the loading capacity and encapsulation efficiency increased with the increase of the DS. The release of insulin slowed as the insulin content increased. The increase of MW or DS of CMCS could slow the release of insulin.
在本研究中，制备了基于羧甲基壳聚糖（CMCS）的自组装囊泡用于胰岛素递送。研究了由CMCS和十六烷基三甲基溴化铵（CTAB）组成的混合体系的自组装行为。所得的CMCS/CTAB囊泡形状近似球形，大多数直径在100nm左右。通过甲基噻唑基四氮唑（MTT）法在体外评估了CMCS/CTAB囊泡的毒性特征。结果表明，通过增加CMCS的取代度（DS）或降低CMCS的分子量（MW）可提高CMCS/CTAB囊泡的稳定性。此外，我们观察到囊泡稳定性随温度降低而提高。胰岛素载药量和包封率随MW增加而降低。而且，载药量和包封率随DS的增加而提高。胰岛素释放随着胰岛素含量的增加而减缓。CMCS的MW或DS的增加可减缓胰岛素的释放。
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