Dourado Lays Fernanda Nunes, Silva Vieira Fernanda Aparecida, Inoue Thomas Toshio, Fialho Silvia Ligorio, de Melo Lutiana Amaral, Pereira Vinicius Viana, de Simoni Juliana Rios, Siman Matheus Soares, de Almeida Cunha Paulo Ferrara, da Silva Cunha Júnior Armando
Faculdade de Farmácia, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
Diretoria de Pesquisa e Desenvolvimento, Fundação Ezequiel Dias, Belo Horizonte, Minas Gerais, Brazil.
J Mater Sci Mater Med. 2025 Jun 9;36(1):49. doi: 10.1007/s10856-024-06845-4.
Changes in collagen orientation and distribution on the corneas lead to the development of diseases characterized by progressive thinning, such as keratoconus. Part of people diagnosed with keratoconus require a corneal graft, which has availability as a major limiting factor. In this scenario, new approaches have been tested to obtain substitute tissues. Porcine cornea has been receiving increasing attention due to its ease of obtaining, biomechanical properties similar to those of human tissue and lower antigenicity. Based on this, the objective of this study was to evaluate the biocompatibility of porcine stroma decellularized by sodium dodecyl sulfate (SDS) through interlamellar implantation in rabbit corneas. The obtained results showed that the lenticule intrastromal implantation was successfully performed and did not elicit rejection. Furthermore, the implanted stroma was able to promote an increase in the thickness of the host cornea. Microscopic analyses revealed that the tissue was well-adhered and the collagen fibrils were more aligned on its periphery. Therefore, it is concluded that the implantation of decellularized porcine stroma occurred satisfactorily and represents a promising alternative to replace human tissue.
角膜中胶原蛋白方向和分布的变化会导致以渐进性变薄为特征的疾病的发展，如圆锥角膜。部分被诊断为圆锥角膜的患者需要进行角膜移植，而角膜供体的可获得性是一个主要限制因素。在这种情况下，人们已经测试了新的方法来获取替代组织。猪角膜因其易于获取、生物力学特性与人体组织相似且抗原性较低而受到越来越多的关注。基于此，本研究的目的是通过层间植入兔角膜来评估经十二烷基硫酸钠（SDS）脱细胞处理的猪角膜基质的生物相容性。所得结果表明，成功进行了透镜状基质内植入，且未引发排斥反应。此外，植入的基质能够促进宿主角膜厚度增加。显微镜分析显示，组织黏附良好，其周边的胶原纤维排列更整齐。因此，得出结论，脱细胞猪角膜基质的植入效果良好，是替代人体组织的一种有前景的选择。
