文献检索文档翻译深度研究
Suppr Zotero 插件Zotero 插件
邀请有礼套餐&价格历史记录

新学期，新优惠

限时优惠:9月1日-9月22日

30天高级会员仅需29元

1天体验卡首发特惠仅需5.99元

了解详情
不再提醒
插件&应用
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
高级版
套餐订阅购买积分包
AI 工具
文献检索文档翻译深度研究
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2025

脂肪组织间充质干细胞条件培养基对掺杂二氧化钛纳米颗粒诱导炎症的抗炎/抗氧化特性

Anti-Inflammatory/Antioxidant Features of Adipose Tissue Mesenchymal Stem Cells Conditioned Media for Doped TiO Nanoparticles in Induced Inflammation.

作者信息

Abd-Rabou Ahmed A, Youssef Ahmed M, El-Khonezy Mohamed I, Kotob Soheir E, Kishta Mohamed S

机构信息

Hormones Department, Medical Research and Clinical Studies Institute, National Research Centre, Dokki, Cairo, 12622, Egypt.

Stem Cell Lab., Center of Excellence for Advanced Sciences, National Research Centre, Dokki, Cairo, 12622, Egypt.

出版信息

ChemistryOpen. 2025 Aug;14(8):e202500261. doi: 10.1002/open.202500261. Epub 2025 Jun 9.

DOI:10.1002/open.202500261
PMID:40489075
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12368888/
Abstract

The fundamental purpose of this work is to determine the anti-inflammatory/antioxidant activity of stem cell conditioned medium enhanced by mono- or dual-doped TiO nanoparticles. The transmission electron microscope, X-ray diffraction, and energy-dispersive X-ray analyses are used to characterize the nanoparticles. Isolation/characterization of adipose tissue mesenchymal stem cells (AD-MSCs) is done. In vitro assays reveal that protein denaturation and proteinase induction are significantly increased with lipopolysaccharide (LPS) comparable to control, while treatments significantly decrease the induction compared to LPS. In vitro assays reveal decreasing in hydroxyl radical scavenging and DPPH radical scavenging activities with LPS, while treatments significantly increase the activity compared to LPS. Induction with LPS decreases in vitro catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx) expression levels and enzyme activities are significantly compared to controls, while treatments significantly increase the CAT expression compared to LPS. Induction with LPS increases the in vitro interleukin 4 (IL4), IL6, IL10, and tumor necrosis factor α expression levels, and their activities significantly decline with treatment compared to controls, while treatments significantly decline expression compared to LPS. The anti-inflammatory and antioxidant properties of AD-MSC-conditioned medium enhanced with mono- or dual-doped TiO nanoparticles are identified in this investigation. To sum up, the work has shown that mono- and dual-doped TiO can inhibit the inflammation caused by LPS in vitro.

摘要

这项工作的基本目的是确定单掺杂或双掺杂二氧化钛纳米颗粒增强的干细胞条件培养基的抗炎/抗氧化活性。使用透射电子显微镜、X射线衍射和能量色散X射线分析对纳米颗粒进行表征。完成了脂肪组织间充质干细胞（AD-MSCs）的分离/表征。体外试验表明，与对照相比，脂多糖（LPS）显著增加了蛋白质变性和蛋白酶诱导，而与LPS相比，处理显著降低了诱导。体外试验表明，LPS使羟自由基清除和DPPH自由基清除活性降低，而与LPS相比，处理显著提高了活性。与对照相比，LPS诱导使体外过氧化氢酶（CAT）、超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GPx）表达水平和酶活性显著降低，而与LPS相比，处理显著提高了CAT表达。LPS诱导增加了体外白细胞介素4（IL4）、IL6、IL10和肿瘤坏死因子α表达水平，与对照相比，其活性在处理后显著下降，而与LPS相比，处理显著降低了表达。本研究确定了单掺杂或双掺杂二氧化钛纳米颗粒增强的AD-MSC条件培养基的抗炎和抗氧化特性。总之，这项工作表明，单掺杂和双掺杂二氧化钛可以在体外抑制LPS引起的炎症。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df67/12368888/c84dac25608b/OPEN-14-e202500261-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df67/12368888/fe7351eea1e8/OPEN-14-e202500261-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df67/12368888/3db114c26347/OPEN-14-e202500261-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df67/12368888/16a90f1269f5/OPEN-14-e202500261-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df67/12368888/6c5f19330f55/OPEN-14-e202500261-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df67/12368888/b3d033099b96/OPEN-14-e202500261-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df67/12368888/9fc0a75163da/OPEN-14-e202500261-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df67/12368888/59c6e442def0/OPEN-14-e202500261-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df67/12368888/cbe85e35bbab/OPEN-14-e202500261-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df67/12368888/a982986c605d/OPEN-14-e202500261-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df67/12368888/c84dac25608b/OPEN-14-e202500261-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df67/12368888/fe7351eea1e8/OPEN-14-e202500261-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df67/12368888/3db114c26347/OPEN-14-e202500261-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df67/12368888/16a90f1269f5/OPEN-14-e202500261-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df67/12368888/6c5f19330f55/OPEN-14-e202500261-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df67/12368888/b3d033099b96/OPEN-14-e202500261-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df67/12368888/9fc0a75163da/OPEN-14-e202500261-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df67/12368888/59c6e442def0/OPEN-14-e202500261-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df67/12368888/cbe85e35bbab/OPEN-14-e202500261-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df67/12368888/a982986c605d/OPEN-14-e202500261-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df67/12368888/c84dac25608b/OPEN-14-e202500261-g007.jpg

相似文献

[1]
Anti-Inflammatory/Antioxidant Features of Adipose Tissue Mesenchymal Stem Cells Conditioned Media for Doped TiO Nanoparticles in Induced Inflammation.

ChemistryOpen. 2025-8

[2]
Mesenchymal stem cells and their secretome modulate stress and enhance lipogenesis in bovine mammary epithelial cells.

Stem Cell Res Ther. 2025-6-23

[3]
Extracellular vesicles derived from clonal mesenchymal stromal cells preconditioned by indirect hypoxia modulate immune responses in diabetic mice more effectively than directly preconditioned vesicles.

Stem Cell Res Ther. 2025-8-26

[4]
Comparative study of adipose tissue derived mesenchymal stem cells with rapamycin on paraquat-induced acute lung injury and pulmonary fibrosis in a mouse model: histological and biochemical study.

Stem Cell Res Ther. 2025-7-15

[5]
Conditioned medium from adipose mesenchymal stromal cells stimulated with pituitary adenylate cyclase-activating polypeptide (PACAP) mitigates RINm5F pancreatic β-cell dysfunction.

Mol Biol Rep. 2025-7-9

[6]
Endothelial cell-derived extracellular vesicles modulate the therapeutic efficacy of mesenchymal stem cells through IDH2/TET pathway in ARDS.

Cell Commun Signal. 2024-5-27

[7]
Adipose-Derived Mesenchymal Stem Cells and Their Derived Epidermal Progenitor Cells Conditioned Media Ameliorate Skin Aging in Rats.

Tissue Eng Regen Med. 2024-8

[8]
The Characterization and Identification of Cyperus Protein: An In Vitro Study on Its Antioxidant and Anti-Inflammatory Potential.

Nutrients. 2025-8-14

[9]
Transplantation of Exercise-Enhanced Mesenchymal Stem Cells Improves Obesity and Glucose Tolerance via Immune Modulation in Adipose Tissue.

Stem Cell Rev Rep. 2025-4-14

[10]
Mesenchymal stem cell-secreted KGF ameliorates acute lung injury via the Gab1/ERK/NF-κB signaling axis.

Cell Mol Biol Lett. 2025-7-10

引用本文的文献

[1]
Exosome mediated delivery of Epigallocatechin 3 gallate as a novel approach to alleviate psoriasis symptoms through cytokine and apoptotic pathway modulation.

Sci Rep. 2025-8-16

本文引用的文献

[1]
Exploring the tumor-suppressive role of miRNA-200c in head and neck squamous cell carcinoma: Potential and mechanisms of exosome-mediated delivery for therapeutic applications.

Transl Oncol. 2025-1

[2]
Generation of cardiomyocytes from stem cells cultured on nanofibrous scaffold: Experimental approach for attenuation of myocardial infarction.

Tissue Cell. 2024-8

[3]
Copper/Tin Nanocomposites-Loaded Exosomes Induce Apoptosis and Cell Cycle Arrest at G0/G1 Phase in Skin Cancer Cell Line.

Chem Biodivers. 2024-8

[4]
Therapeutic Potential of Adipose-Derived Stem Cell-Conditioned Medium and Extracellular Vesicles in an In Vitro Radiation-Induced Skin Injury Model.

Int J Mol Sci. 2023-12-7

[5]
Bone marrow stem cell-derived β-cells: New issue for diabetes cell therapy.

Tissue Cell. 2024-2

[6]
Anti-oxidative activities and anti-ferroptosis of conditioned medium from umbilical cord mesenchymal stem cells.

In Vitro Cell Dev Biol Anim. 2023-10

[7]
Anti-Inflammatory and Antioxidant Activity of Titanium Dioxide Nanotubes Conjugated with Quercetin.

Chem Biodivers. 2023-11

[8]
Introduction to stem cells.

Prog Mol Biol Transl Sci. 2023

[9]
Penfluroidol Attenuates the Imbalance of the Inflammatory Response by Repressing the Activation of the NLRP3 Inflammasome and Reduces Oxidative Stress via the Nrf2/HO-1 Signaling Pathway in LPS-Induced Macrophages.

Mediators Inflamm. 2023

[10]
Mesenchymal Stem Cell Conditioned Medium Modulates Inflammation in Tenocytes: Complete Conditioned Medium Has Superior Therapeutic Efficacy than Its Extracellular Vesicle Fraction.

Int J Mol Sci. 2023-6-29

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

推荐工具

医学文档翻译智能文献检索