Huang Guan-Jiang, Tang Na, Fan Zhi-Jun, Luo Meng-Si, Lu Biao-Qing
Department of Otorhinolaryngology-Head and Neck Surgery, Zhongshan Hospital of Traditional Chinese Medicine, Affiliated to Guangzhou University of Chinese Medicine, Zhongshan, Guangdong, China.
Department of Anesthesiology, Zhongshan Hospital of Traditional Chinese Medicine, Affiliated to Guangzhou University of Chinese Medicine, Zhongshan, Guangdong, China.
Medicine (Baltimore). 2025 Jun 6;104(23):e42662. doi: 10.1097/MD.0000000000042662.
This study investigates the causal relationships between circulating levels of 14 circulating micronutrients and the risk of nasopharyngeal carcinoma (NPC) using Mendelian randomization (MR) analysis. We conducted a 2-sample MR study using genome-wide association studies datasets. Data on 14 circulating micronutrients were obtained from UK Biobank datasets. The genome-wide association studies dataset of NPC (malignant neoplasm of nasopharynx) was downloaded from the FinnGen database. MR analyses were performed using the inverse variance weighted method, the MR-Egger method, and the weighted median method, with sensitivity analyses including MR-Egger, MR-PRESSO, and leave-one-out analyses to test for pleiotropy and heterogeneity. Using the inverse variance weighted method, MR univariate analysis showed that vitamin C, vitamin D, and zinc may have a causal relationship with the risk of NPC. Subsequently, MR multivariate analysis indicated that vitamin C (odds ratio: 1.55 × 1061, 95% confidence interval: 8.52 × 1011-2.81 × 10110, P = .015) and vitamin D (odds ratio: 8.62 × 10-10, 95% confidence interval: 1.73 × 10-17-0.043, P = .021) have a causal relationship with the risk of NPC. Sensitivity analyses confirmed no evidence of directional pleiotropy or significant heterogeneity, supporting the robustness of the findings. Our results provides evidence for causal relationships between circulating levels of vitamin C and vitamin D and the risk of NPC. Our findings underscore the importance of maintaining adequate vitamin D levels to potentially reduce NPC risk, while highlighting the need for caution regarding vitamin C supplementation in at-risk populations. Further research is essential to clarify the mechanisms underlying these relationships.
本研究采用孟德尔随机化（MR）分析方法，探讨14种循环微营养素的循环水平与鼻咽癌（NPC）风险之间的因果关系。我们使用全基因组关联研究数据集进行了一项两样本MR研究。关于14种循环微营养素的数据来自英国生物银行数据集。鼻咽癌（鼻咽恶性肿瘤）的全基因组关联研究数据集从芬兰基因数据库下载。使用逆方差加权法、MR-Egger法和加权中位数法进行MR分析，敏感性分析包括MR-Egger、MR-PRESSO和留一法分析，以检验多效性和异质性。使用逆方差加权法，MR单变量分析表明维生素C、维生素D和锌可能与NPC风险存在因果关系。随后，MR多变量分析表明维生素C（比值比：1.55×1061，95%置信区间：8.52×1011 - 2.81×10110，P = 0.015）和维生素D（比值比：8.62×10-10，95%置信区间：1.73×10-17 - 0.043，P = 0.021）与NPC风险存在因果关系。敏感性分析证实没有方向性多效性或显著异质性的证据，支持了研究结果的稳健性。我们的结果为循环维生素C和维生素D水平与NPC风险之间的因果关系提供了证据。我们的研究结果强调了维持足够维生素D水平以潜在降低NPC风险的重要性，同时突出了对高危人群补充维生素C时需谨慎的必要性。进一步的研究对于阐明这些关系背后的机制至关重要。
