Hoffmann F, Harnisch J P, Bill A
Albrecht Von Graefes Arch Klin Exp Ophthalmol. 1977 Mar 29;202(1):9-18. doi: 10.1007/BF00496764.
Trabeculo-electropuncture (TEP) was performed in one eye of ten cynomolgus monkeys. In five control eyes, the TEP probe was inserted into Schlemm's canal and withdrawn without causing a spark discharge. In another five control eyes,a scleral window was produced and the canal was opened, but no probe was inserted. The gross facility of outflow was determined prior to the operation and 2, 6, and 18 weeks postoperatively. TEP had no longlasting effect on the facility. Gonioscopy five months after the operation revealed marked changes in the anatomy of Schlemm's canal in treated eyes and in control eyes subjected to probe insertion. Control eyes without probe insertion appeared normal. Six months postoperatively three monkeys were killed and the eyes investigated. Light and electron microscopy indicated that insertion of the probe with or without spark discharge resulted in the formation of dense bridges between the inner and outer walls of Schlemm's canal. At the sites of TEP, the trabecular meshwork was replaced by dense scar tissue containing irregular, fine fibrillar material, elastic fibres and large amounts of curly collagen. There were no intertrabecular spaces in the scar tissue. In one eye, one lesion was covered by a thin endothelium resting on a basal membrane. The results indicate that the failure of TEP in monkey eyes was due to the formation of dense scar tissue occluding the openings initially produced by the spark discharge.
在10只食蟹猴的一只眼睛上进行小梁电穿刺（TEP）。在5只对照眼中，将TEP探头插入施莱姆管然后拔出，未引发火花放电。在另外5只对照眼中，制作了巩膜窗并打开了管，但未插入探头。在手术前以及术后2周、6周和18周测定房水流出的总体易度。TEP对易度没有持久影响。术后5个月的前房角镜检查显示，治疗眼和插入探头的对照眼中施莱姆管的解剖结构有明显变化。未插入探头的对照眼外观正常。术后6个月处死3只猴子并对眼睛进行检查。光镜和电镜检查表明，无论有无火花放电，探头的插入都会导致施莱姆管内壁和外壁之间形成致密桥。在TEP部位，小梁网被致密的瘢痕组织取代，瘢痕组织含有不规则的细纤维物质、弹性纤维和大量卷曲的胶原。瘢痕组织中没有小梁间隙。在一只眼睛中，一个病变被一层位于基底膜上的薄内皮覆盖。结果表明，TEP在猴眼中失败是由于形成了致密的瘢痕组织，阻塞了最初由火花放电产生的开口。
