Gurley B J, Buice R G, Sidhu P
Ther Drug Monit. 1985;7(3):321-3. doi: 10.1097/00007691-198507030-00016.
A reversed-phase high performance liquid chromatographic method is presented by which the calcium channel blocker, nifedipine (NFP), may be measured in human serum using 17-alpha-ethinylestradiol as an internal standard. A mobile phase of phosphate buffer (0.01 M, pH 6.1)/methanol/acetonitrile (20:35:45) passed through a muBondapak C-18 column at 1.0 ml/min produced symmetrical bands for nifedipine and internal standard. A rapid and simple chloroform extraction of NFP from 1 ml serum samples proved to be efficient and reproducible. Recovery over a concentration range of 5-100 ng/ml was 92.3 +/- 5.1% (mean +/- SD, n = 6). Ultraviolet absorbance detection at 235 nm was sensitive to serum NFP concentrations of 5 ng/ml. Between-day coefficients of variation at 100 and 5 ng/ml were 4.0 and 11.4%, respectively (n = 10). Serum concentration data from NFP-treated subjects are presented.
本文介绍了一种反相高效液相色谱法，该方法可使用17-α-乙炔基雌二醇作为内标物，测定人血清中的钙通道阻滞剂硝苯地平（NFP）。以磷酸盐缓冲液（0.01 M，pH 6.1）/甲醇/乙腈（20:35:45）为流动相，以1.0 ml/min的流速通过μBondapak C-18柱，硝苯地平和内标物均产生对称峰。结果表明，采用氯仿从1 ml血清样本中快速简单地提取NFP，效果良好且具有可重复性。在5-100 ng/ml的浓度范围内，回收率为92.3±5.1%（平均值±标准差，n = 6）。在235 nm处进行紫外吸光度检测，对血清中5 ng/ml的NFP浓度敏感。在100 ng/ml和5 ng/ml时，日间变异系数分别为4.0%和11.4%（n = 10）。文中还给出了接受NFP治疗的受试者的血清浓度数据。
