Hu Jia-Wei, Pan Yan-Zhi, Zhang Xiao-Xiao, Li Jiang-Tao, Jin Yun
Department of Hepatic-Biliary-Pancreatic Surgery, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310009, Zhejiang Province, China.
World J Gastroenterol. 2025 May 28;31(20):106747. doi: 10.3748/wjg.v31.i20.106747.
Hepatobiliary and pancreatic (HBP) cancers are among the most aggressive malignancies, with recurrence and metastasis driven by tumor heterogeneity and drug resistance, presenting considerable challenges to effective treatment. Currently, personalized and accurate treatment prediction models for these cancers are lacking. Patient-derived organoids (PDOs) tumor are three-dimensional models created from the tumor tissues of individual patients. Recent reports and our cultivation data indicate that the success rate of cultivating organoids for HBP cancers consistently exceeds 70%. The predictive accuracy of these tumor organoids has been shown to surpass 90%. However, PDOs still face notable limitations, especially in simulating the tumor microenvironment, including tumor angiogenesis and the surrounding cellular context, which require further refinement. While co-culture techniques and microfluidic platforms have been developed to mimic multi-cellular environments and functional vascular perfusion, they remain insufficient in accurately recapitulating the complexities of the environment. Additionally, PDOs are needed to fully assess their potential in predicting the efficacy of multi-drug combination therapies. This review provides an overview of the applications, challenges, and prospects for organoid models in the study of HBP cancer.
肝胆胰（HBP）癌是最具侵袭性的恶性肿瘤之一，肿瘤异质性和耐药性导致复发和转移，给有效治疗带来了巨大挑战。目前，缺乏针对这些癌症的个性化且准确的治疗预测模型。患者来源的类器官（PDO）肿瘤是由个体患者的肿瘤组织创建的三维模型。最近的报告和我们的培养数据表明，HBP癌类器官的培养成功率始终超过70%。这些肿瘤类器官的预测准确率已被证明超过90%。然而，PDO仍然面临显著限制，特别是在模拟肿瘤微环境方面，包括肿瘤血管生成和周围细胞环境，这需要进一步完善。虽然已经开发了共培养技术和微流控平台来模拟多细胞环境和功能性血管灌注，但它们在准确再现环境的复杂性方面仍然不足。此外，需要PDO来全面评估它们在预测多药联合疗法疗效方面的潜力。本综述概述了类器官模型在HBP癌研究中的应用、挑战和前景。
