Yin Haiaolong, Hu Jianhua, Li Yaxian, Feng Yi, Li Zhiwei, Sun Rongyan, Tian Lei, Li Ye
Faculty of Food Science and Engineering, Kunming University of Science and Technology, Kunming, 650500, People's Republic of China.
Department of Orthopedic Surgery, the First People's Hospital of Yunnan Province, the Affiliated Hospital of Kunming University of Science and Technology, Kunming, 650500, People's Republic of China.
J Inflamm Res. 2025 Jun 6;18:7323-7336. doi: 10.2147/JIR.S522638. eCollection 2025.
Acute lung injury (ALI) is a serious clinical respiratory disease, but there are few effective drugs for ALI. As a natural product, is used as a drink and medicine in ethnic minority areas of Southwest China. In previous studies, we found that Vaniot methanol extract (EBE) was effective against ALI. This study was carried to investigate the role and mechanisms of EBE ethyl acetate extract (EBEE) against oxidative stress in ALI.
By multiple bioinformatics analysis, AGER and ITGB2 were found to be the potential target genes of EBEE against oxidative stress in ALI. Different doses of EBEE were feed into ALI mice in vivo (600 mg/kg bw, 200 mg/kg bw) and .RAW 264.7 cells (100 μg/mL, 25 μg/mL) in vitro to evaluate the protection function. Western Blot, immunofluorescence and biochemical factor detection were used to measure the levels of oxidative stress associated factors, and gut microbiota analysis examined the gut microbiota in mice.
EBEE, which contained a total of 1374 components, was identified as the most active against oxidative stress. The EBEE treatment most significantly downregulated the ROS, MDA and pathological damages. AGER and ITGB2 were picked up as target genes of EBEE against ALI by bioinformatics. In vitro and in vivo, AGER, NOX4, and P-GSK3β expression levels were significantly decreased, and ITGB2, P-AKT, NRF2 and HO-1 were significantly up-regulated throughout EBEE treatment. By consuming EBEE, significantly increased, while the pernicious bacteria significantly reduced in ALI mouse.
EBEE treatment significantly ameliorated the pathological damage and oxidative stress by regulating AGER, ITGB2 and related pathways in vitro and in vivo. Furthermore, EBEE treatment improved the composition of the gut microbiota in ALI mice. These data suggested that EBEE could be used as a potential functional food for prevention and treatment of ALI.
急性肺损伤（ALI）是一种严重的临床呼吸系统疾病，但针对ALI的有效药物较少。作为一种天然产物，[具体物质未给出名称]在中国西南少数民族地区用作饮品和药物。在先前的研究中，我们发现[具体物质未给出名称]瓦氏甲醇提取物（EBE）对ALI有效。本研究旨在探讨EBE乙酸乙酯提取物（EBEE）在ALI中对抗氧化应激的作用及机制。
通过多种生物信息学分析，发现AGER和ITGB2是EBEE在ALI中对抗氧化应激的潜在靶基因。将不同剂量的EBEE分别用于体内ALI小鼠（600 mg/kg体重，200 mg/kg体重）和体外RAW 264.7细胞（100 μg/mL，25 μg/mL），以评估其保护作用。采用蛋白质免疫印迹法、免疫荧光法和生化因子检测法测量氧化应激相关因子的水平，并通过肠道微生物群分析检测小鼠的肠道微生物群。
共鉴定出含有1374种成分的EBEE对氧化应激最具活性。EBEE处理最显著地下调了活性氧（ROS）、丙二醛（MDA）水平及病理损伤。通过生物信息学分析，AGER和ITGB2被确定为EBEE对抗ALI的靶基因。在体外和体内，整个EBEE处理过程中，AGER、NADPH氧化酶4（NOX4）和磷酸化糖原合成酶激酶3β（P-GSK3β）的表达水平显著降低，而ITGB2、磷酸化蛋白激酶B（P-AKT）、核因子E2相关因子2（NRF2）和血红素加氧酶-1（HO-1）显著上调。通过摄入EBEE，[有益菌未明确给出名称]显著增加，而ALI小鼠体内的有害菌[未明确给出名称]显著减少。
EBEE处理通过在体外和体内调节AGER、ITGB2及相关通路，显著改善了病理损伤和氧化应激。此外，EBEE处理改善了ALI小鼠肠道微生物群的组成。这些数据表明EBEE可作为预防和治疗ALI的潜在功能性食品。
