Salvador-Lujan Gina, Cruz-Pio Liz Erika, Calla Hedersson, Rivera-Asencios Damaris, Solís-Cayo Luis, García-de-la-Guarda Ruth
Laboratorio de Microbiología del Hospital Militar Central «Crl. Luis Arias Schreiber», Lima, Perú.
Laboratorio de Ecología Microbiana, Facultad de Ciencias Biológicas, Universidad Nacional Mayor de San Marcos, Lima, Perú.
Rev Peru Med Exp Salud Publica. 2025 Jun 9;42(1):76-81. doi: 10.17843/rpmesp.2025.421.13818.
Pseudomonas aeruginosa is an opportunistic pathogen associated with health care infections, it has high levels of antimicrobial resistance and is associated with hospital outbreaks. Early outbreak detection is a usual problem in hospitals, therefore, this study aimed to assess the clonal relationship of carbapenemase-producing P. aeruginosa in a tertiary hospital in Lima, Peru. Twenty-four metallo β-lactamase-producing P. aeruginosa strains isolated from hospitalized patients were collected. The clonal relation was determined using the REP-PCR technique. REP-PCR band profiles were normalized, analyzed and combined using BioNumerics version 7.6 software. Molecular identification showed 19 different profiles and four clonal groups. We determined polyclonality among isolates. We did not find clonal dissemination among the metallo-β-lactamase-producing P. aeruginosa strains circulating in the hospital. Motivation for the study. The isolation of carbapenemase-producing Pseudomonas aeruginosa in different wards of a tertiary care hospital prompted the identification of the clonality of the isolates and to determine whether they corresponded to an intrahospital outbreak. Main findings. The REP-PCR technique grouped the 24 strains of metallo-β-lactamase-producing P. aeruginosa isolated from patients in different hospital wards into 19 profiles. The greatest clonal diversity was found in the medical ward. Public health implications. Molecular typing by REP-PCR could be a practical and rapid alternative for the surveillance and control of hospital outbreaks.
铜绿假单胞菌是一种与医疗保健感染相关的机会致病菌，它具有高水平的抗菌耐药性且与医院暴发有关。早期暴发检测是医院中常见的问题，因此，本研究旨在评估秘鲁利马一家三级医院中产碳青霉烯酶的铜绿假单胞菌的克隆关系。收集了从住院患者中分离出的24株产金属β-内酰胺酶的铜绿假单胞菌菌株。使用REP-PCR技术确定克隆关系。使用BioNumerics 7.6版软件对REP-PCR条带图谱进行标准化、分析和合并。分子鉴定显示有19种不同的图谱和4个克隆组。我们确定了分离株之间的多克隆性。我们在医院中传播的产金属β-内酰胺酶的铜绿假单胞菌菌株中未发现克隆传播。研究动机。在一家三级护理医院的不同病房中分离出产碳青霉烯酶的铜绿假单胞菌，促使对分离株的克隆性进行鉴定，并确定它们是否对应于医院内的暴发。主要发现。REP-PCR技术将从不同医院病房的患者中分离出的24株产金属β-内酰胺酶的铜绿假单胞菌菌株分为19种图谱。在内科病房中发现了最大的克隆多样性。公共卫生意义。通过REP-PCR进行分子分型可能是监测和控制医院暴发的一种实用且快速的替代方法。
