一种用于通过高效液相色谱法测定猪组织中磺胺二甲嘧啶的快速样品制备方法。

A rapid sample preparation method for the determination of sulfamethazine in swine tissues by high-performance liquid chromatography.

作者信息

Haagsma N, Nooteboom R J, Gortemaker B G, Maas M J

出版信息

Z Lebensm Unters Forsch. 1985 Sep;181(3):194-7. doi: 10.1007/BF02425576.

Abstract

A rapid procedure for the determination of sulfamethazine in swine muscle and kidney is described. The method comprises sonication-aided extraction with dichloromethane, cleaning-up of the extract on a combination of silica and reversed-phase C18 Sep-Pak cartridges and analysis by high-performance liquid chromatography on a Hypersil ODS column using acetonitrile/ammonium acetate solution (10 mmol X 1(-1), pH 6.8) 1 + 3 v/v as the mobile phase. Detection was performed at 254 nm. Mean recoveries of added sulfamethazine from swine muscle and kidney, at levels of 0.05 to 0.5 micrograms X g-1, were 89.5 and 80.5%, respectively. After intramuscular injection of sulfamethazine into swine, residues in tissues were also determined. The application of ultrasonic energy for the extraction of sulfamethazine residues from animal tissues is also discussed.

摘要

本文描述了一种快速测定猪肌肉和肾脏中磺胺二甲嘧啶的方法。该方法包括用二氯甲烷进行超声辅助提取，在硅胶和反相C18 Sep-Pak柱组合上对提取物进行净化，并在Hypersil ODS柱上用乙腈/醋酸铵溶液（10 mmol·L⁻¹，pH 6.8）1 + 3 v/v作为流动相通过高效液相色谱进行分析。检测波长为254 nm。在添加水平为0.05至0.5 μg·g⁻¹时，猪肌肉和肾脏中添加的磺胺二甲嘧啶的平均回收率分别为89.5%和80.5%。向猪肌肉注射磺胺二甲嘧啶后，还测定了组织中的残留量。本文还讨论了利用超声能量从动物组织中提取磺胺二甲嘧啶残留的应用。