使用PCR-CRISPR/Cas12a系统检测粪便样本中的[此处原文似乎缺失关键信息]
Detection of in fecal samples using PCR-CRISPR /Cas12a system.
作者信息
Zheng Yan, Liu BiXia, Zuo QianFei, Deng Fei, Lan ChunHui
机构信息
Department of Gastroenterology, Chongqing Key Laboratory of Digestive Malignancies, Daping Hospital, Army Medical University (Third Military Medical University), Chongqing, PR China.
College of Medicine, Southwest Jiaotong University, Chengdu, PR China.
出版信息
Bioanalysis. 2025 Jun;17(11):737-745. doi: 10.1080/17576180.2025.2518042. Epub 2025 Jun 12.
OBJECTIVE
To develop a highly sensitive and specific detection method based on PCR-CRISPR/Cas12a for the detection of () in feces and to evaluate its detection rate in the general population as well as its potential as a gastrointestinal tumor marker.
MATERIALS AND METHODS
Specific primers and crRNA targeting the 16S rDNA of were designed to construct a PCR-CRISPR/Cas12a detection system. A total of 230 fecal samples were collected from the general population, and bacterial DNA was extracted. The target gene was detected using this system to verify its sensitivity, specificity, and stability.
RESULTS
The established detection system demonstrated strong specificity, with stable recognition of , and a minimum detection limit of 10 ng/μL. The detection rate of in fecal samples from the general population was 51.7%.
CONCLUSION
The PCR-CRISPR/Cas12a system can efficiently detect in feces, providing a reliable technical tool for exploring its association with gastrointestinal tumors.
目的
开发一种基于PCR-CRISPR/Cas12a的高灵敏度和特异性检测方法，用于检测粪便中的（），并评估其在普通人群中的检测率以及作为胃肠道肿瘤标志物的潜力。
材料与方法
设计靶向（）16S rDNA的特异性引物和crRNA，构建PCR-CRISPR/Cas12a检测系统。从普通人群中收集了230份粪便样本，提取细菌DNA。使用该系统检测目标基因，以验证其灵敏度、特异性和稳定性。
结果
所建立的检测系统具有很强的特异性，对（）识别稳定，最低检测限为10 ng/μL。普通人群粪便样本中（）的检测率为51.7%。
结论
PCR-CRISPR/Cas12a系统可有效检测粪便中的（），为探索其与胃肠道肿瘤的关联提供了可靠的技术工具。
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