Garbett Krassimira, Allen James, Sando Richard C
Department of Pharmacology, Vanderbilt Brain Institute, Vanderbilt University, Nashville, TN 37240 USA.
bioRxiv. 2025 Jun 3:2025.06.02.657384. doi: 10.1101/2025.06.02.657384.
Neural circuits composed of multitudes of diverse synaptic connections self-organize during mammalian brain development. A balance between excitatory and inhibitory synaptic function is required for information processing by these neural circuits. Despite the importance of this balance, the interplay between excitatory and inhibitory synaptic assembly during circuit establishment remains unclear due to a lack of means to monitor both processes simultaneously. Here, we develop imaging approaches to visualize and track excitatory and inhibitory synapses concurrently. By applying these approaches, we find that despite continual dynamics, excitatory and inhibitory synaptic density remain at synchronized levels during synaptogenesis. These results support coordinated excitatory and inhibitory synapse assembly to maintain functional balance despite continual synaptic turnover.
由大量多样的突触连接组成的神经回路在哺乳动物大脑发育过程中会自我组织。这些神经回路进行信息处理需要兴奋性和抑制性突触功能之间的平衡。尽管这种平衡很重要，但由于缺乏同时监测这两个过程的手段，在回路建立过程中兴奋性和抑制性突触组装之间的相互作用仍不清楚。在这里，我们开发了成像方法来同时可视化和追踪兴奋性和抑制性突触。通过应用这些方法，我们发现尽管存在持续的动态变化，但在突触形成过程中兴奋性和抑制性突触密度仍保持同步水平。这些结果支持兴奋性和抑制性突触组装的协同作用，以在突触持续更新的情况下维持功能平衡。
