[过氧乙酸对芽孢杆菌孢子杀灭率的测定]

[Determination of the killing rate of Bacillus spores by peracetic acid].

作者信息

Botzenhart K, Jaax R

出版信息

Zentralbl Bakteriol Mikrobiol Hyg B. 1985 Jun;181(1-2):139-50.

Abstract

The sporocidal properties of peracetic acid (PAA) at defined concentrations were characterized by determination of decrease in PAA after addition of D-glucose, human albumin and suspensions of spores; concentration of PAA, which inactivates 10(6)-10(7) spores of Bacillus cereus, B. subtilis, B. megaterium and B. licheniformis within 10-30 min; inactivation constant k, decimal reduction time D and the sporocidal index (mg PAA X min X ml-1) at that concentration. In contrast to albumin, the spore suspension caused relatively little reduction of PAA concentration (less than 5% at the concentrations used). B. cereus, B. subtilis and B. megaterium had similar inactivation rates with k-values in the range of 0.368 and 0.541 min-1, D-values between 4.26 min and 6.26 min at 0.2 mg/ml PAA. B. lichenformis was much more resistant showing a k = 0.345 min-1 and D = 6.66 min at 3.0 mg/ml PAA. The sporocidal index of B. lichenformis was 180 mg X min X ml-1 while the three other species had sporocidal index-values of 7 mg X min X ml-1.

摘要

通过测定添加D-葡萄糖、人白蛋白和孢子悬液后过氧乙酸（PAA）的减少量，来表征特定浓度下过氧乙酸的杀孢子特性；确定在10 - 30分钟内使蜡样芽孢杆菌、枯草芽孢杆菌、巨大芽孢杆菌和地衣芽孢杆菌的10(6)-10(7)个孢子失活的PAA浓度；失活常数k、十进制减少时间D以及该浓度下的杀孢子指数（mg PAA×min×ml-1）。与白蛋白不同，孢子悬液导致PAA浓度的降低相对较少（在所使用的浓度下小于5%）。蜡样芽孢杆菌、枯草芽孢杆菌和巨大芽孢杆菌具有相似的失活速率，k值在0.368至0.541 min-1范围内，在0.2 mg/ml PAA时D值在4.26分钟至6.26分钟之间。地衣芽孢杆菌的抗性要强得多，在3.0 mg/ml PAA时k = 0.345 min-1，D = 6.66分钟。地衣芽孢杆菌的杀孢子指数为180 mg×min×ml-1，而其他三个物种的杀孢子指数值为7 mg×min×ml-1。