Ren Le, Liu Jun, Xu Ya-Yun, Shi Zhen-Wang
Department of Gastroenterology, Second People's Hospital of Hefei, Hefei 230011, Anhui Province, China.
Department of Ophthalmology, The Third People's Hospital of Hefei, Hefei 230011, Anhui Province, China.
World J Clin Oncol. 2025 May 24;16(5):107551. doi: 10.5306/wjco.v16.i5.107551.
Multiple lines of evidence have indicated that pro-inflammatory cytokines play a role in the pathophysiology of gastric carcinoma (GC).
To identify potential serum cytokine-based biomarkers for GC diagnosis.
The study cohort comprised 50 patients diagnosed with GC and 50 healthy control subjects. A panel of 7 pro-inflammatory cytokines, including interleukin (IL)-1β, IL-2, IL-6, IL-8, IL-12, tumor necrosis factor-α, and interferon-γ (IFN-γ) were quantified using multiplex Luminex assays. Comparative analyses were conducted to evaluate cytokine levels between the GC patients and healthy controls. The diagnostic potential of serum pro-inflammatory cytokines in differentiating GC patients from healthy individuals was assessed through receiver operating characteristic (ROC) curve analysis. The correlation between serum cytokine levels and disease severity, as classified by the tumor-node-metastasis staging system, was analyzed using Spearman's rank correlation coefficient.
In comparison to the control group, patients with GC demonstrated significantly elevated serum levels of IL-1β ( = -4.089, < 0.001), IL-6 ( = -3.983, < 0.001), IL-8 ( = -5.460, < 0.001), and IFN-γ ( = -2.856, = 0.005). ROC curve analysis indicated that the area under the curve values for IL-1β, IL-6, and IL-8 exceeded 0.7, effectively distinguishing GC patients from healthy controls. Additionally, serum levels of IL-1β ( = 0.424, = 0.012) and IL-6 ( = 0.742, < 0.001) were positively correlated with the T stage in GC patients. Similarly, serum concentrations of IL-1β ( = 0.356, = 0.039) and IL-6 ( = 0.441, = 0.008) exhibited a positive association with the N stage in these patients.
These findings suggest that circulating pro-inflammatory cytokines, such as IL-1β, IL-6, and IL-8, may serve as potential biomarkers for the diagnosis of GC.
多条证据表明促炎细胞因子在胃癌（GC）的病理生理学中起作用。
确定基于血清细胞因子的潜在生物标志物用于胃癌诊断。
研究队列包括50例诊断为胃癌的患者和50名健康对照者。使用多重Luminex检测法对一组7种促炎细胞因子进行定量，包括白细胞介素（IL）-1β、IL-2、IL-6、IL-8、IL-12、肿瘤坏死因子-α和干扰素-γ（IFN-γ）。进行比较分析以评估胃癌患者和健康对照者之间的细胞因子水平。通过受试者工作特征（ROC）曲线分析评估血清促炎细胞因子在区分胃癌患者和健康个体中的诊断潜力。使用Spearman等级相关系数分析血清细胞因子水平与根据肿瘤-淋巴结-转移分期系统分类的疾病严重程度之间的相关性。
与对照组相比，胃癌患者血清中IL-1β（ = -4.089， < 0.001）、IL-6（ = -3.983， < 0.001）、IL-8（ = -5.460， < 0.001）和IFN-γ（ = -2.856， = 0.005）水平显著升高。ROC曲线分析表明，IL-1β、IL-6和IL-8的曲线下面积值超过0.7，可有效区分胃癌患者和健康对照者。此外，胃癌患者血清IL-1β（ = 0.424， = 0.012）和IL-6（ = 0.742， < 0.001）水平与T分期呈正相关。同样，这些患者血清IL-1β（ = 0.356， = 0.039）和IL-6（ = 0.441， = 0.008）浓度与N分期呈正相关。
这些发现表明，循环促炎细胞因子，如IL-1β、IL-6和IL-8，可能作为胃癌诊断的潜在生物标志物。
