Lu Kun, Ding He, Long Mengmeng, Zhu Xiaoyang, Lou Xiaoyi, Li Yan, Gu Ning
Jiangsu Key Laboratory for Biomaterials and Devices, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210009, P. R. China.
Nanjing Key Laboratory for Cardiovascular Information and Health Engineering Medicine, Institute of Clinical Medicine, Nanjing Drum Tower Hospital, Medical School, Nanjing University, Nanjing 210093, P. R. China.
ACS Appl Mater Interfaces. 2025 Jun 25;17(25):36511-36520. doi: 10.1021/acsami.5c07229. Epub 2025 Jun 12.
Given that reactive oxygen species (ROS) accumulation and inflammatory microenvironments are key issues hindering wound repair, nanozyme-functionalized hydrogels have been widely used in different types of wound healing modes to regulate ROS. Since wound healing is a long-term process, the long-term effectiveness or even enhancement of enzyme-like activities of hydrogels is critical. Based on the electron transfer catalytic mechanism and enzyme-like activity enhancement effect of Prussian blue nanozymes (PBNZs), this study used classic hydrogel materials as carriers and PBNZs as functionalized nanozymes to construct a wound dressing with enhanced enzyme-like activity. In the system, the effect of self-enhanced enzyme activity as a wound dressing and the good promotion effect on the healing of skin defect models were successfully verified. Moreover, PBNZs can be stably fixed in the hydrogel system and will not be released during the application process, which is a necessary condition for achieving enzyme-like activity self-enhancement. It is precisely due to the self-enhanced effect of its enzyme-like activity that PBNZs can express excellent ROS regulation ability in the effective contact area. This article explores the intrinsic properties of PBNZs-based hydrogels for wound healing, focusing on their catalytic mechanisms. The developed PBNZs-functionalized hydrogel shows significant potential as an advanced dressing to accelerate wound healing. Notably, the self-enhanced wound dressing with enzyme-like activity proposed in this study offers valuable insights for material design in this field.
鉴于活性氧（ROS）积累和炎症微环境是阻碍伤口修复的关键问题，纳米酶功能化水凝胶已被广泛应用于不同类型的伤口愈合模式以调节ROS。由于伤口愈合是一个长期过程，水凝胶类酶活性的长期有效性甚至增强至关重要。基于普鲁士蓝纳米酶（PBNZs）的电子转移催化机制和类酶活性增强效应，本研究以经典水凝胶材料为载体，PBNZs为功能化纳米酶，构建了具有增强类酶活性的伤口敷料。在该体系中，成功验证了作为伤口敷料的自增强酶活性效应以及对皮肤缺损模型愈合的良好促进作用。此外，PBNZs可以稳定地固定在水凝胶体系中，在应用过程中不会释放，这是实现类酶活性自增强的必要条件。正是由于其类酶活性的自增强效应，PBNZs能够在有效接触区域表现出优异的ROS调节能力。本文探讨了基于PBNZs的水凝胶用于伤口愈合的内在特性，重点关注其催化机制。所开发的PBNZs功能化水凝胶作为一种加速伤口愈合的先进敷料具有巨大潜力。值得注意的是，本研究提出的具有类酶活性的自增强伤口敷料为该领域的材料设计提供了有价值的见解。
