Gimeno-Martos Silvia, Gómez-León Alicia, Luigia Bosa, Lorenzo Pedro L, María Arias-Álvarez, Rosa María García-García, Rebollar Pilar G
Dept. of Physiology, Veterinary Faculty, Complutense University of Madrid, Av. Puerta de Hierro s/n, 28040, Madrid, Spain; Dept. of Biochemistry and Molecular and Cell Biology, Veterinary Faculty Zaragoza University, 50013, Zaragoza, Spain.
Dept. of Physiology, Veterinary Faculty, Complutense University of Madrid, Av. Puerta de Hierro s/n, 28040, Madrid, Spain.
Theriogenology. 2025 Oct 1;245:117524. doi: 10.1016/j.theriogenology.2025.117524. Epub 2025 Jun 6.
Mammalian reproductive function is regulated by hypothalamic neurons that secrete Kisspeptin (Kp), a neuropeptide that stimulates gonadotropin-releasing hormone (GnRH) secretion, triggering pituitary gonadotrophins (LH and FSH) and gonadal steroids. This study evaluated the effect of Kisspeptin-10 (Kp10) on ovulation induction in rabbits, comparing its efficacy with that of the GnRH analogue gonadorelin. Multiparous New Zealand White × California does were assigned to three groups: control group (0.5 % saline solution, i.v.), GnRH group (20 μg gonadorelin, i.m.), and Kp10 group (250 μg Kp10, i.v.). Kp10 induced ovulation in 87.5 % of does, matching the response observed in the GnRH group, with a comparable number of corpora lutea (CL) per ovulated doe (12.9 ± 1.4 vs 14.6 ± 1.4 CL/doe, respectively). On day 7, plasma progesterone (P4) was significantly higher in ovulated GnRH-treated does than in Kp10-treated ones (25.12 ± 4.17 vs 9.47 ± 4.17 ng/mL; p < 0.0211), while non-ovulated controls exhibited minimal P4 concentrations (0.86 ± 0.12 ng/mL). Plasma estradiol (E2) levels showed no significant differences across days or groups, with mean values of 32.74 ± 4.33 pg/mL on day 0 and 37.27 ± 5.49 pg/mL on day 7, respectively. Histological analysis confirmed that Kp10 promoted preovulatory follicle development and CL formation, mirroring GnRH effects. Additionally, Kp10 enhanced angiogenesis, indicated by increased vascular endothelial growth factor (VEGF) expression in more developed follicles and CL. These results suggest that Kp10 could be an alternative to GnRH for ovulation induction in rabbits, although further studies are needed to explore optimal analogues, doses, and administration routes.
哺乳动物的生殖功能受下丘脑神经元调节，这些神经元分泌促性腺激素释放激素（GnRH）刺激因子——吻素（Kp），一种刺激促性腺激素释放激素（GnRH）分泌的神经肽，进而触发垂体促性腺激素（LH和FSH）以及性腺类固醇的分泌。本研究评估了吻素-10（Kp10）对兔排卵诱导的影响，并将其疗效与GnRH类似物戈那瑞林进行比较。经产新西兰白兔×加利福尼亚母兔被分为三组：对照组（静脉注射0.5%盐溶液）、GnRH组（肌肉注射20μg戈那瑞林）和Kp10组（静脉注射250μg Kp10）。Kp10诱导了87.5%的母兔排卵，与GnRH组观察到的反应相当，每只排卵母兔的黄体（CL）数量相当（分别为12.9±1.4个与14.6±1.4个CL/母兔）。在第7天，经GnRH处理排卵的母兔血浆孕酮（P4）显著高于经Kp10处理的母兔（25.12± 4.17 vs 9.47± 4.17 ng/mL；p<0.0211），而未排卵的对照组P4浓度极低（0.86±0.12 ng/mL）。血浆雌二醇（E2）水平在各天或各组之间无显著差异，第0天和第7天的平均值分别为32.74±4.33 pg/mL和37.27±5.49 pg/mL。组织学分析证实，Kp10促进了排卵前卵泡发育和CL形成，与GnRH的作用相似。此外，Kp10增强了血管生成，这在发育更成熟的卵泡和CL中血管内皮生长因子（VEGF）表达增加得到体现。这些结果表明，Kp10可能是兔排卵诱导中GnRH的替代物，尽管需要进一步研究以探索最佳类似物、剂量和给药途径。
