Gameiro Gustavo Rosa, Valente Carolina C, Augsburger James J, Correa Zelia M
Department of Ophthalmology, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, FL 33136, USA.
Centro Universitario Lusiadas, Faculdade de Ciencias Medicas de Santos, Santos 11045-101, SP, Brazil.
J Clin Med. 2025 May 23;14(11):3650. doi: 10.3390/jcm14113650.
: This study aims to evaluate whether fine-needle aspiration biopsy (FNAB) of melanocytic uveal tumors (MUTs) using 27-gauge (27G) needles yields aspirates like those obtained using 25-gauge (25G) needles for cytology. : A retrospective review was conducted on 32 primary uveal melanomas (PUMs). Tumors were sampled at three adjacent sites, first using a 27G needle for gene expression profile (GEP) testing, second and third with 27G and 25G needles for cytology. The endpoints evaluated were the sufficiency of aspirates for cytopathology and GEP. : Among the 32 patients, 17 tumors were choroidal, 6 ciliochoroidal, 7 iridociliochoroidal, and 2 exclusively iridic. Tumor diameter ranged from 3.3 mm to 23 mm (mean 13.2 mm), and thickness ranged from 0.5 mm to 12 mm (mean 6.4 mm). Aspirates from both needle sizes were sufficient for cytopathological diagnosis and GEP in 31 of 32 cases (96.9%). The single insufficient aspirate was insufficient with both the 27G and 25G needles. The cytopathology was identical in all other cases. The tumors were Class 1 in 22 cases (71.0%) and Class 2 in 9 cases (29.0%). : FNAB aspirates of MUTs using 27G needles appear sufficient for cytology and GEP in most cases, showing a similar diagnostic yield compared to 25G needles.
本研究旨在评估使用27号（27G）针进行葡萄膜黑素细胞肿瘤（MUTs）的细针穿刺活检（FNAB）所获得的吸出物是否与使用25号（25G）针进行细胞学检查所获得的吸出物相似。
对32例原发性葡萄膜黑色素瘤（PUMs）进行了回顾性研究。在三个相邻部位对肿瘤进行采样，首先使用27G针进行基因表达谱（GEP）检测，第二和第三个部位分别使用27G和25G针进行细胞学检查。评估的终点是吸出物用于细胞病理学和GEP的充分性。
在32例患者中，17例肿瘤位于脉络膜，6例位于睫状体脉络膜，7例位于虹膜睫状体脉络膜，2例仅位于虹膜。肿瘤直径范围为3.3 mm至23 mm（平均13.2 mm），厚度范围为0.5 mm至12 mm（平均6.4 mm）。在32例病例中的31例（96.9%）中，两种针型获得的吸出物均足以进行细胞病理学诊断和GEP检测。唯一一次吸出物不足的情况是使用27G针和25G针时均不足。在所有其他病例中，细胞病理学结果相同。22例（71.0%）肿瘤为1级，9例（29.0%）为2级。
在大多数情况下，使用27G针进行MUTs的FNAB吸出物似乎足以进行细胞学检查和GEP检测，与25G针相比，诊断率相似。
