Qian Enze, Eltawila Ahmed, Kang Yunqing
Department of Ocean & Mechanical Engineering, Florida Atlantic University, Boca Raton, FL 33431, USA.
Department of Biomedical Engineering, Florida Atlantic University, Boca Raton, FL 33431, USA.
Int J Mol Sci. 2025 May 26;26(11):5092. doi: 10.3390/ijms26115092.
Effective osteogenesis for bone regeneration is still considerably challenging for a porous β-tricalcium phosphate (β-TCP) scaffold to achieve. To overcome this challenge, hollow manganese dioxide (H-MnO) nanoparticles with an urchin-like shell structure were prepared and added in the porous β-TCP scaffold. A template-casting method was used to prepare the porous H-MnO/β-TCP scaffolds. As a control, solid manganese dioxide (S-MnO) nanoparticles were also added into β-TCP scaffolds. Human bone mesenchymal stem cells (hBMSC) were seeded in the porous scaffolds and characterized through cell viability assay and alkaline phosphatase (ALP) assay. Results from in vitro protein loading and releasing experiments showed that H-MnO can load significantly higher proteins and release more proteins compared to S-MnO nanoparticles. When they were doped into β-TCP, MnO nanoparticles did not significantly change the surface wettability and mechanical properties of porous β-TCP scaffolds. In vitro cell viability results showed that MnO nanoparticles promoted cell proliferation in a low dose, but inhibited cell growth when the added concentration went beyond 0.5%. At a range of lower than 0.5%, H-MnO doped β-TCP scaffolds promoted the early osteogenesis of hBMSCs. These results suggested that H-MnO in the porous β-TCP scaffold has promising potential to stimulate osteogenesis. More studies would be performed to demonstrate the other functions of urchin-like H-MnO nanoparticles in the porous β-TCP.
对于多孔β-磷酸三钙（β-TCP）支架而言，实现有效的骨再生成骨仍然具有相当大的挑战性。为了克服这一挑战，制备了具有海胆状壳结构的中空二氧化锰（H-MnO）纳米颗粒，并将其添加到多孔β-TCP支架中。采用模板铸造法制备多孔H-MnO/β-TCP支架。作为对照，还将固体二氧化锰（S-MnO）纳米颗粒添加到β-TCP支架中。将人骨髓间充质干细胞（hBMSC）接种到多孔支架中，并通过细胞活力测定和碱性磷酸酶（ALP）测定进行表征。体外蛋白质加载和释放实验结果表明，与S-MnO纳米颗粒相比，H-MnO能够加载显著更多的蛋白质并释放更多的蛋白质。当它们掺杂到β-TCP中时，MnO纳米颗粒并没有显著改变多孔β-TCP支架的表面润湿性和力学性能。体外细胞活力结果表明，MnO纳米颗粒在低剂量下促进细胞增殖，但当添加浓度超过0.5%时会抑制细胞生长。在低于0.5%的范围内，H-MnO掺杂的β-TCP支架促进了hBMSC的早期成骨。这些结果表明，多孔β-TCP支架中的H-MnO具有刺激成骨的潜在前景。将进行更多研究以证明海胆状H-MnO纳米颗粒在多孔β-TCP中的其他功能。
