Lv Wen, Wang Ruiyue, Ji Wenbo, Fu Benzhong, Luo Ming, Han Jian
Department of Plant Pathology, College of Agronomy, Xinjiang Agricultural University, Urumqi 830052, China.
Key Laboratory of Prevention and Control of Invasive Alien Species in Agriculture and Forestry of the North-Western Desert Oasis (Co-Construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Urumqi 830052, China.
Plants (Basel). 2025 May 22;14(11):1564. doi: 10.3390/plants14111564.
Fire blight, a devastating bacterial disease caused by , has posed significant challenges to apple and pear production for over a century. This study introduces a gliding filamentous bacterium, the strain NSD29, isolated from natural forest soil in Xinjiang, China, as a biological control agent for managing this disease. Comprehensive characterization based on morphological, physiological, biochemical, 16S rRNA gene, and whole-genome analyses identified the strain NSD29 as . The train NSD29 demonstrated potent predatory activity against in vitro. Its biocontrol efficacy was subsequently evaluated on detached leaves, inflorescences, young fruit, and shoots of fragrant pear under controlled greenhouse conditions. Results indicated that applying . NSD29 significantly inhibited lesion expansion on pear leaves and young fruit, achieving protective efficacies of 75.2% and 72.0%, respectively. Furthermore, pre-treatment spraying with NSD29 effectively reduced the incidence of blossom blight, with a control efficacy of 61.2%. On detached pear shoots, the application of NSD29 fermentation broth suppressed lesion expansion, demonstrating substantial protective (86.8%) and curative (75.6%) efficacies. This research provides the first evidence for the potential of species in the biological control of plant diseases, highlighting NSD29 as a promising candidate for developing strategies to combat fire blight.
火疫病是一种由[病原菌名称缺失]引起的毁灭性细菌性病害，在一个多世纪以来一直给苹果和梨的生产带来重大挑战。本研究介绍了一种从中国新疆天然森林土壤中分离出的滑行丝状细菌NSD29菌株，作为防治这种病害的生物防治剂。基于形态学、生理学、生物化学、16S rRNA基因和全基因组分析的综合表征将NSD29菌株鉴定为[具体菌种名称缺失]。NSD29菌株在体外对[病原菌名称缺失]表现出强大的捕食活性。随后在可控温室条件下，对香梨的离体叶片、花序、幼果和嫩枝评估了其生物防治效果。结果表明，施用NSD29可显著抑制梨树叶和幼果上病斑的扩展，分别达到75.2%和72.0%的保护效果。此外，用NSD29进行预处理喷雾可有效降低花腐病的发病率，防治效果为61.2%。在离体梨嫩枝上，施用NSD29发酵液可抑制病斑扩展，表现出显著的保护效果（86.8%）和治疗效果（75.6%）。本研究首次证明了[菌种名称缺失]物种在植物病害生物防治中的潜力，突出了NSD29作为开发防治火疫病策略的有前途的候选菌株。
