Zhong Fanjia, Wang Fengpan, Chen Zike, Huang Tengbo, Zhao Panpan
Guangdong Provincial Key Laboratory for Plant Epigenetics, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518060, China.
The Orchid Conservation and Research Center of Shenzhen, Shenzhen 518060, China.
Plants (Basel). 2025 May 25;14(11):1613. doi: 10.3390/plants14111613.
The R2R3-MYB transcription factor GAMYB plays crucial roles in plant growth and development, but the biological functions of in tomato remain poorly understood. Here, we investigated the roles of by overexpressing a miR159-resistant version () in tomato. Transgenic plants exhibited a dwarf phenotype with reduced internode elongation, which was associated with decreased bioactive gibberellin (GA) levels due to transcriptional repression of and activation of . Additionally, altered leaf morphology by inhibiting cell proliferation through downregulation of cell cycle genes, resulting in larger but fewer epidermal cells. Intriguingly, plants displayed increased floral organ number, a process likely mediated by the upregulation of rather than GA signaling. These findings demonstrate that regulates diverse aspects of tomato development through both GA-dependent and independent pathways, providing new insights into the functional diversification of genes and potential strategies for genetic improvement of tomato architecture and yield.
R2R3-MYB转录因子GAMYB在植物生长发育中起关键作用，但在番茄中的生物学功能仍知之甚少。在此，我们通过在番茄中过表达抗miR159的版本（）来研究其作用。转基因植物表现出矮化表型，节间伸长减少，这与生物活性赤霉素（GA）水平降低有关，这是由于的转录抑制和的激活所致。此外，通过下调细胞周期基因抑制细胞增殖，从而改变了叶片形态，导致表皮细胞更大但数量更少。有趣的是，植物的花器官数量增加，这一过程可能是由的上调而非GA信号介导的。这些发现表明，通过GA依赖和独立途径调节番茄发育的多个方面，为基因的功能多样化以及番茄株型和产量遗传改良的潜在策略提供了新的见解。
